MES (C, H13NO,S) s commonly used as a buffer in biology and biochemistry. It has a pk, of 6.15 at 20°C. Calculate the mass (in g) of MES (HA in the Henderson-Hasselbalch equation) needed to obtain 100 mL of a 0.100 M MES buffer (total buffer concentration) with a pH of 5.9. Note: Although you will use different buffer systems and a different pH in lab, this is the same math that you will be using for lab. Report your answer to three places after the decimal.

Chemistry & Chemical Reactivity
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ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
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Chapter17: Principles Of Chemical Reactivity: Other Aspects Of Aqueous Equilibria
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Problem 108IL: A buffer solution with it pH of 12.00 consists of Na3PO4 and Na2HPO4. The volume of solution is...
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MES (C, H13 NO,S) is commonly used as a buffer in biology and biochemistry. It has a pk, of 6.15 at
20°C.
Calculate the mass (in g) of MES (HA in the Henderson-Hasselbalch equation) needed to obtain 100
ml of a 0.100 M MES buffer (total buffer concentration) with a pH of 5.9.
Note: Although you will use different buffer systems and a different pH in lab, this is the same math that you
will be using for lab. Report your answer to three places after the decimal.
Transcribed Image Text:MES (C, H13 NO,S) is commonly used as a buffer in biology and biochemistry. It has a pk, of 6.15 at 20°C. Calculate the mass (in g) of MES (HA in the Henderson-Hasselbalch equation) needed to obtain 100 ml of a 0.100 M MES buffer (total buffer concentration) with a pH of 5.9. Note: Although you will use different buffer systems and a different pH in lab, this is the same math that you will be using for lab. Report your answer to three places after the decimal.
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