Mixtures of amino acids can be analyzed by first separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. For each pair of amino acids listed, determine which will be eluted first from the cation-exchange column by a pH 7.0 buffer. (a) Asp and Ly, (b) Arg and Met, (c) Glu and Val, (d) Gly and Leu, (e) Ser and Ala

Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
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Chapter2: Life's Chemical Basis
Section: Chapter Questions
Problem 4CT: Some undiluted acids are not as corrosive as when they are diluted with water. That is why lab...
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2. Mixtures of amino acids can be analyzed by first separating the mixture into its components
through ion-exchange chromatography. Amino acids placed on a cation-exchange resin
containing sulfonate (-SO₁) groups flow down the column at different rates because of
two factors that influence their movement: (1) ionic attraction between the sulfonate
residues on the column and positively charged functional groups on the amino acids, and
(2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic
backbone of the polystyrene resin. For each pair of amino acids listed, determine which
will be eluted first from the cation-exchange column by a pH 7.0 buffer. (a) Asp and Ly,
(b) Arg and Met, (c) Glu and Val, (d) Gly and Leu, (e) Ser and Ala
Transcribed Image Text:2. Mixtures of amino acids can be analyzed by first separating the mixture into its components through ion-exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO₁) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. For each pair of amino acids listed, determine which will be eluted first from the cation-exchange column by a pH 7.0 buffer. (a) Asp and Ly, (b) Arg and Met, (c) Glu and Val, (d) Gly and Leu, (e) Ser and Ala
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