Moody [1] studied the efficiency of a GC separation of2-butanone on a dinonyl phthalate column. Evaluatingplate height as a function of flow rate gave a vanDeemter equation for which A is 1.65 mm, B is 25.8 mm*mL/min, and C is 0.0236 mm-min/mL. a) UsingMicrosoft Excel, plot a graph of H versus u for flow ratesin the range of 5 - 120 mL/min. Your graph mustinclude curves due to the contributions of all the terms (i.e. curve for A, B and C and the overall curve) b) Forwhat range of flow rates does each term in the VanDeemter equation have the greatest effect? c) Howmuch is the optimum flow rate for this column? d) Howmuch is the height of a theoretical plate for the flowrate in part C ?

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Moody [1] studied the efficiency of a GC separation of 2-butanone on a dinonyl phthalate column. Evaluating plate height as a function of flow rate gave a van Deemter equation for which A is 1.65 mm, B is 25.8 mm •mL/min, and C is 0.0236 mm-min/mL. a) Using Microsoft Excel, plot a graph of H versus u for flow rates

Moody [1] studied the efficiency of a GC separation of 2-butanone on a dinonyl phthalate column. Evaluating plate height as a function of flow rate gave a van Deemter equation for which A is 1.65 mm, B is 25.8 mm •mL/min, and C is 0.0236 mm-min/mL. a) Using Microsoft Excel, plot a graph of H versus u for flow rates

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.19QAP

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