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1) most STR fragments used in human
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- Which technique used to manipulate genetic material results in a significant increase in DNA or RNA fragments? Group of answer choices heat shock treatment electroporation gel electrophoresis polymerase chain reactionDNA fragments can be separated and identified by(gas chromatography, capillary electrophoresis).The presence of a narrow band between the Beta and gamma regions on SPE would most likely indicate which of the following preanalytical errors had occured? sample was mislabeld, sample was grossly hemolyzed, sample was plasma not serum, sample not protected from light
- a. DNA fingerprinting is a method of visualizing similarities and differences in the DNA sequence of individuals. Arrange the following items so that they show the DNA fingerprinting steps in proper order: - Gel electrophoresis - Stain or probe for DNA - cut DNA into fragments - Amplify DNA sample using PCR b. On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?SDS is required to unfold proteins during SDS-PAGE electrophoresis and it is included in both the loading and running buffer. If SDS is not included in the running buffer.... the proteins in your sample may partially renature thus affecting migration. the gel will not conduct current in the absence of SDS. there is no effect on the samples. the proteins in your sample will be graded.In which reagent is extracted DNA suspended to put it in solution? A. Sodium citrate saline buffer B. Sodium dodecyl sulfate (SDS) C. Cold absolute alcohol D. HCl E. H2SO4
- How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be specific (4-5 lines) .Are Mass Spectrometry and Immunoassay (IA) the most common drug screening methods that are used in labs? . Why are these two methodologies the gold standard for forensic drug testing in crime labs. (don't explain how this methods work)Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). ( NOTE: previous answer said that a,b, and d are true; nevertheless I can choose only one). Need help. A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel. Which of the following is true of the gel electrophoresis shown? Note: Only one can be true... I'm still between c or d. Need help A) the power source has not been turned on yet. B) the three wells contained the same DNA molecules. C) well #1 had fewer molecules of DNA than well #2 or #3 D) well #3 had more different sized molecules of DNA than well #1 or #2
- three criteria are DNA profiling methods evaluated by...... Speed, discriminating power and sensitivity False positive rate, cost, and general acceptance Variety of testable samples, cost, standardization False negative rate, speed and general acceptance Discriminating power, general acceptance, error ratewhat is the incident beam it is the beam of light that exits the sample it is the beam of light is absorbed by the sample it is the initial beam of chemical energy that projected into the sample in a spectrophotometer it is the beam of light that converts light energy into electrical energy in a spectrophotometer it is the initial beam of light that is projected from the light source in a spectrophotometer -wich of the following researches described DNA as helical using X-ray diffraction studies. Maurice Wiking and Rosalind franklin Erwin chargaff and Rosalind franklin James Watson and Francis Crick Francis crick and Albert Kossel Johann mischief and albert kossel beer’s law establishes a direct relationship between absorption and temperature wavelength osmosis concentration pHA probe is a full section of DNA or RNA that has been labeled with a tracer. True False
