n your experiment there will be 4 different experimental samples labeled A-D (please see Table 3 on page 12 of the Lab 4 handout). In sample D, if you saw some bacterial colonies that are blue and some colonies that are white, what would this tell you? Group of answer choices the sgRNA was completely non functional the CRISPR/Cas9 donor DNA repair is not 100% efficient there is no functional lacZ on this bacterial plate arabinose was not active on this bacterial plate

n your experiment there will be 4 different experimental samples labeled A-D (please see Table 3 on page 12 of the Lab 4 handout). In sample D, if you saw some bacterial colonies that are blue and some colonies that are white, what would this tell you? Group of answer choices the sgRNA was completely non functional the CRISPR/Cas9 donor DNA repair is not 100% efficient there is no functional lacZ on this bacterial plate arabinose was not active on this bacterial plate

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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In your experiment there will be 4 different experimental samples labeled A-D (please see Table 3 on page 12 of the Lab 4 handout). If you saw no bacterial colonies growing in sample A (pLZDonor, no arabinose), what would this tell you? Group of answer choices the bacteria has been successfully edited to include the donor template DNA the sgRNA caused a double-strand DNA break which caused the bacteria to die the DNA transformation was not successful there is functional lacZ in this sample
The following DNA sequence is from a bacteriophage that infects a pathogenic bacterium and scientists want to know if this bacteriophage could prove to be a potential treatment against it. But first scientists need to discover if different strains of this pathogen have restriction endonucleases that it may use for its own protection. They try 3 different RE’s:a) EcoR1 b) HaeIII c) BamH1 Look up the recognition sequences for the 3 Res. Enzymes above and check whether the phage genome (a snippet of which is shown below) will or will not be ‘cut’. Tell me how their experiment worked out and what their conclusion was.G A A A A G G C C A C A A G G C C G T C G A C T T T T A A A A G G C C A C A T G C G G C T T T T C C G G T G T T C C G G C AG C T GA A A AT T T T C C G G T G T A C G CC
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? A.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. B.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. C.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors D.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. E. Cas cuts directly into palindromic restriction sites that it targets in the DNA.
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.
If a researcher wanted to utilize CRISPR/Cas9 to target a gene of interest in their experimental system (i.e. Drosophila) which of the following would they customize for this purpose Group of answer choices Guiding region of sgRNA None of the above, CRISPR can only function in bacterial cells Cas9 enzyme Scaffold region of sgRNA
Choose 1 or more than 1 RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with a molecular weight marker. The separated RNA fragments are then transferred to an RNA-binding membrane. Next, this membrane is incubated with labelled probe specific for the gene X. This experiment determines: how many copies of gene X there are in liver cells. if the gene X is translated in liver cells. if gene X has a point mutation in liver cells. the chromosomal location of gene X. the length of the transcript of gene X.
Hello, is this correct? What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a gene? Check All That Apply Cas1 geneCas1 gene Cas9 geneCas9 gene sgRNAsgRNA donor DNAdonor DNA bacteriophage DNA
Which of the following statements is not true about the guide RNA (see this interactive demonstration for help with this question: https://www.biointeractive.org/classroom-resources/crispr-cas9-mechanism-applications) Group of answer choices it contains a sequence of 20 nucleotides that matches a specific sequence in a cell’s DNA When the guide RNA is combined with Cas9, it will guide Cas9 to the target sequence its target sequence can be almost any sequence as long as it occurs near a PAM motif it is a nuclease, a type of enzyme that cleaves DNA
Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?
Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infection
Need help Your friend wants to make a mutation to disable the ACE2 gene (this gene encodes the protein that SARS-CoV-2 uses to enter human cells) in cultured human cells but doesn’t know how. Explain to her how she could use CRISPR to do this. You answer should explain how the ACE2 gene is targeted, cut, and mutated.
Cas9 is a protein that cuts nucleic acids a delivery vector a small DNA binding protein clusters of short palindromic DNA