Given what we've discussed in class, what will be most likely outcome if you conjugate an streptomycin resistant ampicillin sensitive methionine auxotroph E. coli strain (engineered to be pir+) that is F- with a streptomycin sensitive non-HFR methionine prototroph strain that is F- and RP4+ but contains pUC18?

Given what we've discussed in class, what will be most likely outcome if you conjugate an streptomycin resistant ampicillin sensitive methionine auxotroph E. coli strain (engineered to be pir+) that is F- with a streptomycin sensitive non-HFR methionine prototroph strain that is F- and RP4+ but contains pUC18?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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. a. You want to perform an interrupted-mating mappingwith an E. coli Hfr strain that is Pyr+, Met+, Xyl+,Tyr+, Arg+, His+, Mal+, and Strs. Describe anappropriate bacterial strain to be used as theother partner in this mating.b. In an Hfr × F− cross, the pyrE gene enters therecipient in 5 minutes, but at this time point thereare no exconjugants that are Met+, Xyl+, Tyr+,Arg+, His+, or Mal+. The mating is now allowed toproceed for 30 minutes and Pyr+ exconjugants areselected. Of the Pyr+ cells, 32% are Met+, 94% areXyl+, 7% are Tyr+, 59% are Arg+, 0% are His+, and71% are Mal+. What can you conclude about theorder of the genes?
By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Explain how these results are consistent with the idea that thebacterial chromosome is circular?
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In your laboratory, you have an F− strain of E. coli that is resistantto streptomycin and is unable to metabolize lactose, but it can metabolizeglucose. Therefore, this strain can grow on a medium thatcontains glucose and streptomycin, but it cannot grow on a mediumcontaining lactose. A researcher has sent you two E. colistrains in two separate tubes. One strain, let’s call it strain A, hasan F′ factor (an F prime factor) that carries the genes that are requiredfor lactose metabolism. On its chromosome, it also has thegenes that are required for glucose metabolism. However, it is sensitiveto streptomycin. This strain can grow on a medium containinglactose or glucose, but it cannot grow if streptomycin is addedto the medium. The second strain, let’s call it strain B, is an F−strain. On its chromosome, it has the genes that are required forlactose and glucose metabolism. Strain B is also sensitive to streptomycin.Unfortunately, when strains A and B were sent to you, thelabels had fallen…
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You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown on the right, what is the order of these four genes along the bacterial chromosome? Explain your reasoning.
By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample oftheir experimental results is shown in the following table: A. E xplain how these results are consistent with the idea that thebacterial chromosome is circular.B. Draw a map of the bacterial chromosome that shows the orderof genes and the locations of the origins of transfer among thesedifferent Hfr strains.
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By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?
Consider the five E. coli merodiploid strains listed here. Strain #1 I+P+O+Z-Y+/I+P+OcZ+Y+ Strain #2 I+P+O+Z+Y+/I-P+OcZ+Y- Strain #3 I+P+O+Z-Y+/I-P+OcZ+Y- Strain #4 I-P-O+Z+Y-/I+P+OcZ-Y+ Strain #5: ISP+O+Z+Y+/I-P+O+Z+Y- Which of these strains will express b-galactosidase constitutively? Select all correct answers. A.)Strain #5 B.)Strain #1 C.)Strain #4 D.)Strain #2 E.)Strain #3