Name of Organism: Enterobacter aerogenes Туре of Color Before Incubation Color After Incubation Color: Positivel Reagents Added? Medium Phenol Red Lactose Negative? Red Reagents Added? (Same for all) Phenol Red to detect Gas Bubble: Phenol Red Glucose Red Color: Gas Bubble: color change Citrate Green Phenol Red to detect color change Bromophenol SIM Clear Sulfur: Blue Indole: Methyl Red (MR) Clear For Indole, we add Kovac's EMB Reddish-no growth
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- Please answer fast What is this organism? And what other test could be done to confirm it's identification? 1. Gram stain - positive cocci, chains Hemolysis- gamma BE - positive NaCl - positive Catalase - weakly positive 2. Gram positive cocci, chains NaCl - negative BE - negative Hippurate - positive Hemoylsis - beta Catalase - negativePlease answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredQuestion:- I have a case (not real btw) of a 25 year old female who was admitted to the hospital with a history of persistent fever that did not respond to amoxicillin or acetaminophen or ibuprofen. She was a resident of the Philippines who had been travelling in Europe for the previous 11 days. On physical examination, she was febrile, had an enlarged liver, abdominal pain, and an abnormal urinalysis. After isolation and cultivation, the infectious agent was found to exist as Gram-negative, facultative anaerobic rods. The organism could fermenter and was oxidase negative. It possessed lipopolysaccharide consisting of outer somatic O polysaccharide, core polysaccharide (common antigen) and lipid A (endotoxin) What is the infectious agent and what further tests could you do to confirm the preliminary identification?
- LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratingsGreetings :) Based on the results of using Hanging-drop method in Figure 2, what microorganisms can you find? Kindly state or identify all microbes you can see, and briefly described each of them. Thank you so much!topic: BACTERIAL ENDOTOXINS TEST Fill-in the possible effect of the procedure and give the rationale. The sample solution prepared exceeded the MVD. Effect: Rationale:
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- Dilution Problem.A culture of Staphylococcus is diluted as follows:(1) 70mL are added to 330mL of water.(2) 100uL from (1) are then added to 9.9mL of water.(3) A 10-3 dilution is made from tube # (2).(4) 10uL from (3) are plated for a pour plate and incubated. There were 80 colonies counted on one half of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?c) How many milliliters of water is needed to make a 10-1 dilution using 100 uL from the original culture?Topic: Microbiological Examination of Non-sterile Products 1. In an indicative test, donut-shaped dark pink colonies appeared. Interpretation: Rationale:Order: penicillin G 1 million units IM Q6HSupply: 5 million unit powderDiluent: 3 mL sterile waterConcentration: 1 million units/mL