Neutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of enzyme is it? Assume Vmax is 35 µM min-1 . When you provide 3.0 x 10-5 M of sphingomyelin, you observe an initial velocity of 6.0 µM min-1. Calculate the KM.
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Neutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of enzyme is it? Assume Vmax is 35 µM min-1
. When you provide 3.0 x 10-5 M of sphingomyelin, you observe an initial velocity of 6.0 µM min-1. Calculate the KM.
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- If instead of using 3.5 µM myoglobin (receptor) you used half of this (that is, 1.75 µM myoglobin), what would be that value of the Kd, that you calculated ( how would it change)? Please explain so I can solve on my own :) (How does changing concentration of the receptor in a ligand-receptor binding experiment affect the dissociation constant?)Describe the effects of a lethal dose of ionizingradiation at the molecular level. What type of filterwould be used to filter sterilize a heat-sensitive liquid?SHOW COMPLETE SOLUTION 1. An electrochemical cell consist of 0.2715 Ibs of MnO2, and 0.2524 Ibs of PbO2. A current flow through a 1.1 ohm resistor of electrochemical cell for 45 mins. The reaction takes place at 400k and the total volume of the solution is 504.9 cm^3. Use the standard reduction cell potential table for MnO2 and PbO2: concentration of Mn2+ (2.81 M) and PbO2 (0.95 M) Cell Potential E=0.48 V current when it passes through a 1.1 ohm resistor = 0.44 ampere Determine the following: a. quantity of electricity/charge, Q b. Energy consumed in Joules c. Power in Kilowatts
- The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A. Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?Calculate the mass of invertase (in mg) and concentration of invertase (in mM) contained in a 25.0mL sample of yeast extract that has 3,000 total units of activity, assuming that pure invertase has a specific activity of 1,000 units/mg with a mass of 270kD.
- In a rat cardiomyocyte, the levels of creatine, phosphocreatine, and free phosphate were found to be 37.6mM, 40.3mM, and 8.02mM, respectively. Given that the standard free energy change is –43.0 kJ/mol, what is the true free energy change for phosphocreatine hydrolysis in the cardiomyocyte described above?A bacterial enzyme catalyzes the hydrolysis of maltose as shown in the reaction given below: Maltose + H2O -> 2 glucose If the reaction has a Km of 0.135 mM and a V max of 65 m mol/min. What is the reaction velocity when the concentration of maltose is 1.0 mM?A 40 umol.l-1 solution of flavin mononucleotide (FMN) gave an absorbance of 0.8 at 470 nm using a 1 cm cuvette. What is the molar extinction coefficient of FMN in M-1 cm-1?
- What is the change in concentration (umol/mL/min) for NADPH if the change in absolute absorbance per second from your DHFR functional assay analysis was 0.0035?Plot a line weaver-burk graph for Km=6.30mM and Vmax=360uM/min when an experiment has 5 tubes with concentrations of substrates of 1.0mM, 10mM, 50mM, and 100mM.If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02 sec/mol, and x-intercept is -2.5 mM-1, then what is the KM value?
