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- Why not just inject the double-stranded cDNA that codes for the spike protein of the virus? What additional step or steps would you need to use to get the human muscle cells to produce the spike protein if the cDNA was injected to serve as the virus? The new Covid19 vaccine produced by two companies (Pfizer, Moderna) uses mRNA coding for part of the spike protein of the virus. The virus uses the spike protein to invade human cells where it replicates. Is it surprising that the mRNA must be stabilized with chemicals that need ultra-cold or frozen storage to protect the mRNA from degradation before it causes human muscle cells to make the spike protein?The same molecular process that causes antigenic drift in HIV can lead to resistance to some antiretroviral drugs used in HIV therapy. Which of the following explains why a combinationdifferent antiretroviral drugs is more effective than a single antiretroviral drug? Select ALL that apply.a).Each antiretroviral drug has a different mechanism of action and targets a different aspect of HIV replication.b).By using 3 different antiretroviral drugs at the same time, it is highly unlikely that a combination of mutations conferring drug resistance will occur at the same time.c)Administering 3 antiretroviral drugs achieves higher levels of each drug than administering just one antiretroviral drug.d).By using 3 different antiretroviral drugs, these drugs are likely to be more effective in inhibiting the same HIV enzyme than one antiretroviral drug.e),Administering 3 antiretroviral drugs is less toxic than administering one antiretroviral drug.The new Covid19 vaccine produced by two companies (Pfizer, Moderna) uses mRNA coding for part of the spike protein of the virus. The virus uses the spike protein to invade human cells where it replicates. Is it surprising that the mRNA must be stabilized with chemicals that need ultra-cold or frozen storage to protect the mRNA from degradation before it causes human muscle cells to make the spike protein? Some Covid 19 vaccines in development use the adenovirus, a common vector used in gene therapies, to inject the mRNa for the spike protein into human muscle cells. Why might vaccines using a virus vector be more likely to need only refrigeration and not deep-cold storage, given what you know about viruses? Some older vaccines used a weakened strain of the pathogenic virus in the injection in the hope that the weakened virus would induce an immune response without causing disease. The virus vectors used in genetic engineering have all the harmful virus genes cut out and replaced with…
- Some older vaccines used a weakened strain of the pathogenic virus in the injection in the hope that the weakened virus would induce an immune response without causing disease. The virus vectors used in genetic engineering have all the harmful virus genes cut out and replaced with the foreign gene, such as one that makes a protein that is absent in the human patient. Which type of viral vaccine would you prefer to take as the safest option? Why? Some Covid 19 vaccines in development use the adenovirus, a common vector used in gene therapies, to inject the mRNa for the spike protein into human muscle cells. Why might vaccines using a virus vector be more likely to need only refrigeration and not deep-cold storage, given what you know about viruses?This is a hypothetical scenario. A recently emerged virus has been transmitting and mutating rapidly in humans throughout the world. If a vaccine that stops transmission of all current circulating variants of the virus in humans is utilized and herd immunity is achieved throughout the world, would this essentially stop 1) further transmission of the virus; 2) further mutation of the virus, respectively? Why/why not? Please answer the question succinctly and in bullet points!A common way to prevent infectious viral diseases, such as COVID-19 and the flu, is to develop a vaccine for the virus that causes them. Vaccines can be made from a virus’s proteins or other parts, or from a virus that has been inactivated and is no longer harmful. Some vaccines, such as the flu vaccine, need to be modified every year to keep up with changes in the virus. Studies have shown that SARS-CoV-2 has a slower mutation rate than the influenza virus, which causes the flu. This means that mutations occur less frequently in SARS-CoV-2’s genome than in the influenza virus’s genome. Based on the information above, explain why it may be possible to develop a SARS-CoV-2 vaccine that would not have to be modified as often as the flu vaccine.
- What are the similarities and differences between retroviruses and retrotransposons? It has been hypothesizedthat retroviruses evolved from retrotransposons. Do youagree with this model? Justify your answerIn 1918, an influenza pandemic that originated with avian flu killed 50 million people. Researchers isolated samples of that virus from bodies of infected people preserved in Alaskan permafrost since 1918. From the samples, they sequenced the viral genome, then reconstructed the virus. The reconstructed virus is 39,000 times more infectious than modern influenza strains, and 100 percent lethal in mice. Understanding how this virus works can help us defend ourselves against other deadly influenza strains that arise. For example, discovering what makes it so infectious would help researchers design more effective vaccines. Critics of the research are concerned: If the virus escapes the containment facilities (even though it has not done so yet), it might cause another pandemic. Worse, terrorists could use the published DNA sequence and methods to make the virus for horrific purposes. Do you think this research makes us more or less safe?You test the same bacteria used to perform the experiment in the graph below for resistance to penicillin and you find that the "%MIC of ancestor" is 95. Is phage treatment better, worse, or about the same, at reducing resistance to penicillin as it is to erythromycin?
- One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?Which of the following is a known characteristic or procesds that can lead to genetic changes in some viruses? When two different viral strains of the same type of virus infect a single individual, reassortment/mixing of segmented genome pieces can occur, resulting in new combinations of segments. Genetic rearrangements of the viral genome occurs as a result of duplicate operons in the viral genome undergoing homologous recombination. Environmental conditions trigger the SOS response in viruses, and leads to generation of errors/mutations in the viral genome. Viral RNA polymerases display proofreading capability in replicating the viral genome of certain viruses.In the lysogenic cycle of bacteriophages, which of the fol-lowing is true?(a) Lysogeny is the term applied to a temperate phage thatdoes not replicate itself independently and does not lysethe bacterial host cell.(b) Phage DNA is incorporated into the host bacterium’sDNA, at which time it is called a prophage.(c) Temperate phages can be replicated either as a pro-phage along with bacterial chromosomal replicationor can suddenly revert to the lytic cycle by replicatingthemselves and assembling into new phages.(d) All the above are true.(e) a and c only.