On paper, replicate the following segment of DNA: (UPLOAD PHOTO OF YOUR ANSWER) 5' ATCGG CTACGITCAC 3' 3' TAGCCGATGCAAGTG5'

Q: What is replica plating?

A:

Q: What is the polypeptide that will be formed from the following DNA sequence?  Template DNA…

A: In molecular biology complementary base pairing or complementarity is a reletion between the two…

Q: Sort the size (bp) of DNA fragments on this gel from small to large. 1 smallest and 4 = largest. B

A: Electrophoresis is the method in which the dispersed particles show motion that is related to the…

Q: A piece of DNA fragment is sequenced. You clone the the fragment, isolate the cloned DNA fragment,…

A: While deciphering a sequencing gel, we know that it depends on terminating the reaction at 3' end…

Q: For the chromatogram below, what is the sequence of the template DNA from base 115 to 125?…

A: According to the question, we have to answer the question that which is for the chromatograph below,…

Q: The synthetic unit of the polymerase chain reaction is the replica. True or false?

A: Introduction  PCR is the revolutionized technique invented by Karry Mullis in 1983, for which he got…

Q: Which protein directs the recombination exchange with a DNA that has failed a complete DNA…

A: DNA unwinds and the two strands detach at the "replication origins" a little at a time, like a…

Q: Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move…

A: Electrophoresis the separation of fragment based on their size and charge.Agarose gel…

Q: During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be…

A: Gel electrophoresis is the technique for the separation of DNA fragments on the basis of size and…

Q: Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with…

A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…

Q: Which protein directs the recombination exchange with a DNA that has failed a complete DNA…

A: The process of recombination in DNA which has been damaged and failed the process of replication…

Q: In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Solid…

A: Okazaki fragments are short stretches of DNA on the lagging strand, which are synthesized in the…

Q: How many different temperatures are used in each cycle of the polymerase chain reaction?

A: Polymerase chain reaction (PCR): It is a molecular technique used to make copies of desired gene.…

Q: To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?a)…

A: The correct option is  CaCl2 (b) CaCl2 is the chemical which is added to prevent degradation of the…

Q: Define the following terms: Genome Bacteriophage λ DNA

A: 1) Genome -  Genome is the whole set of genetic instructions found in a cell. It has all the…

Q: Which of the following is most relevant to the term "hybridization"? a) base pairing b) nuclease c)…

A: Hybridization is to combine two substances to make a hybrid. In molecular biology, Hybridization is…

Q: If one of the DNA strands being replicated “reads” CGAATG, what will be the base sequence of the…

A: DNA(deoxyribonucleic acid) is a double-stranded helical genetic material containing thousands of…

Q: Why do DNA Polymerases Require a Primer?

A: DNA Polymerase DNA Polymerase is the enzyme responsible for normal DNA synthesis. Different kinds of…

Q: 3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI BamHI Ncol EcoR1/BamH1 BamHI/Nco1…

A: Plasmids are double-stranded circular DNA molecules. Due to their small size and stability, these…

Q: Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA…

A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around…

Q: Sanger DNA sequencing/ dideoxy sequencing was used as shown in the diagram below. The arrow…

A: Sanger's sequencing method. It is a method based on DNA synthesis. In normal DNA synthesis,…

Q: Which of the following would represent a section of DNA with a palindromic sequence? 5' TAT CCG 3'…

A: A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule which on…

Q: Which DNA fragment will be closest to the top (negative pole) of anelectrophoretic gel?a. 450 bp b.…

A: DNA can be measured in base pairs. We can consider base pair as basic unit of DNA. 1000 bp = 1 kb (…

Q: Describe the activities of the enzymes required to construct a recombinant DNA molecule.

A: Genetic engineering is the major area for the production of recombinant DNA. The recombinant DNA is…

Q: You transform 500 uL of cells with 2.5 ug of plasmid DNA, and then you take 100 uL and spread that…

A: Answer. Transformation efficiency Transformation efficiency is defined as the number of…

Q: which step of the polymerase chain reaction takes place at 98 degrees?

A: It is an enzymatic process by which specific segments of DNA can be amplified by using a primer,…

Q: In order to replicate both strands of DNA SIMULTANEOUSLY , E. coli bacteria folds or loops one…

A: Replication is the process by which new strands are synthesized complimentary to their parent…

Q: Three pieces of cut DNA were isolated using gel electrophoresis.The DNA that is the longest will be…

A: At first, let's see what is gel electrophoresis? Actually, gel electrophoresis is a type of…

Q: What are the differences between In vitro and In vivo DNA replication?  Please answer at your own…

A: Question: What are the differences between Invitro and Invivo DNA replication? Answer: Invitro DNA…

Q: The diagram shows an autoradiograph of a DNA sequencing gel. What is the 5' to 3' sequence of the…

A: Gel electrophoresis is a technique which is used to separate the molecules based on the size of the…

Q: Ehat  primer sets could be amplify the following DNA sequence?   AATACGTCGCATGGggatccttttttatgcatg

A: Amplification of DNA sequence is done by the polymerase chain reaction. With the help of DNA…

Q: If you have five test tubes each containing a double stand DNA, which of the following double…

A: Answer- The denaturation of DNA is depend on the melting temperature and the composition of the…

Q: Using the following DNA sequence what would be the complementary DNA made during replication? TAC…

A: Complementary DNA  is synthesized from a mature mRNA strand and it lacks both promoters and introns.…

Q: Which of the following DNA sequences will travel the shortest amount down a gel? A ATTGCAGGCCT B…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Why is the use of temperaturestable DNA polymerase an important factor in the polymerase chain…

A: The polymerase chain reaction (PCR) is the process of artificially synthesizing multiple copies of a…

Q: Diagram the steps for the production of recombinant DNA. Label and Identify the steps involved and…

A: In the image provided above, the bacterial plasmid is composed of restriction sites such as EcoRI…

Q: When DNA replication was investigated by using heavy, N15 DNA to mark the original molecules, and…

A: DNA replication of phenomenon of formation of new strand of DNA . There are several model of DNA…

Q: Gel Electrophoresis Separates DNAFragments According to which thing?

A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…

Q: Describe the structural features of DNA that enable it to be replicated.

A: Deoxyribonucleic acid or DNA is a molecule that contains the genetic code of the organisms. DNA…

Q: What is the role of a primer in DNA replication? What would happen if you forgot to add a primer in…

A: DNA replication is the process in which a parent DNA is separated and two new strands are formed…

Q: For the chromatogram below, what is the sequence of the template DNA from base 115 to 125? CTGTGTGAA…

A: DNA is formed of four types of nitrogenous bases, which are adenine, thymine, guanine, and cytocine.…

Q: Using the correct base pairing rules for DNA replication, what would be the complementary strand for…

A: Rules of base pairing A with T: The purine adenine(A)  always pairs with the pyrimidine Thymine…

Q: During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be…

A: The answer is 100 kb .

Q: Following agarose gel electrophoresis, where on the gel will the largest DNA molecules be, and why?…

A: in agarose gel electrophoresis the agarose gel is poured on the electrophoresis plate and wells are…

Q: Draw the gel electrophoretic pattern that would be seen in dideoxy sequenceanalysis of the DNA…

A: Gel Electrophoresis is a separation technique that was used to separate the Biomolecules such as…

Q: 1.1kbp 1.5 kbp Hindll Hindll 0.15 kbp amp 0.25 kbp If you were to digest this plasmid with HindllI,…

A: Plasmids are a small, extrachromosomal segment of DNA(deoxyribonucleic acid) and are mostly found in…

Q: A segment of DNA is isolated and added to a 01 mixture of four deoxynucleotides, two specific…

A: The correct option is D.

Q: In  the DNA model, what are the features that contribute to the stability and the ability of the DNA…

A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.