Our Western Blot experiment requires a buffer called TBS. At working concentration (1X), this buffer contains 20 mM Tris 150 mM NaCl Prior to use, however, the buffer is made and stored as a 10X concentrated stock a What are the concentrations of these two ingredients in the 10X stock? Primary antibodies for the Western Blot experiment are prepared using this TBS buffer, with the addition of several other ingredients Calculate the amount of each ingredient needed to produce10 ml of the final working antibody solutio b 10X TBS stock C001% (V) Tween detergent d 3% (w/y) BSA (molecular weight 66430 g mol) e 1:10 000 dilution of antibody stock

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Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.