Ovalbumin is one of the main proteins in egg white. If you consider a diameter of 5.4486 x 10-9 m and its specific volume of 0.75 cm3 / g. a) Calculate the coefficient of diffusion of ovalbumin in water at 20 ° C b) Determine its molecular weight.
Q: Consider olive oil, an oil with a high percentage of fat derived from oleic acid (otherwise known as…
A: When the two hydrogen atoms of the fatty acids which adjoin the double bond are in the same chain,…
Q: Which nuclear isotope used in protein NMR spectroscopy is the most sensitive to detect? Briefly…
A: NMR Spectroscopy is used to study the structure and composition of biomolecules like proteins. Here…
Q: Calculate the unknown concentration of the PROTEIN E with an absorbance value of A678 given the…
A: Proteins have aromatic amino acids that absorb the maximum light of 280 nm wavelength. This…
Q: There are four proteins present in a solution: Protein A (pl = 5), Protein B (pl = 3), Protein C (pl…
A: Ion exchange chromatography occurs because of the electrostatic attraction existing between charged…
Q: Aside from cholesterol, what other chemical components of the cell membrane contributes to its…
A: Membrane chemistry can be rain on the basis of fluid mosaic model. According to this model a…
Q: What are the components of a lysine buffer at pH 9.2? Refer to the following forms of lysine: A H D…
A: Lysine is a basic amino acid with an amino group in its side chain. Lysine is an important amino…
Q: . Oxygen binding by the hemocyanin of the shrimp Callianassa has been measured. Using the following…
A: A graph will be plotted between log P and log (fraction / 1-fraction) A trend line will be plotted.…
Q: Choose ONE of the bond types listed below which most accurately describes the bonds involved in…
A: Proteins are macromolecules that are made up of amino acids. Amino acids are the basic structural…
Q: A protein molecule is approximately spherical and has a hydrodynamic diameter of 35μm. What is the…
A: The protein interaction is classified as one of the most important and fundamental steps in the…
Q: If an antibody binds its antigen with affinity of 150 nanomolar, what is the standard binding energy…
A: The binding and dissociation interactions between an antibody (Ab) and its antigen (An) is shown…
Q: Describe in detail the vapour diffusion method for crystallising a protein
A: Vapor diffusion is the most typically employed method of protein crystallization. In this method,…
Q: Given a dipeptide Gly-Val, Gly: Pk,=2.34; pkK,=9.60 Val: Pk,=2.32; pK,=9.62 a. draw the protonic…
A: The peptides are biomolecules formed of more than one amino acid joined through an amide bond. The…
Q: A. NAME THE STRUCTURES USING THE STANDARD IUPAC SYSTEM. 1. CH, CH3 -CH- CH-CH, CH, CH,
A: You have asked multiple questions. I will be answering the first question. For structure shown in 1…
Q: Given a tripeptide Cys-His-Lys, Cys: Pk: = 1.71; Pk2= 10.78; Pke= 8.33 His: Pk = 1.82; Pkz= 9.17;…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Consider a negatively charged protein adsorbed on anion-exchange gel at pH 8. (a) How will a…
A: Proteins are essential nutrients for the human body. They are one f the building blocks of body…
Q: whereas protein B does not absorb at 340 nm. What absorbance will be observed when light at 340 nm…
A: Given, ε (extinction coefficient) = 6440 M-1 cm-1 l (path length) = 5 mm = 0.5 cm c (concentration)…
Q: Student A says that he can insert any value of optical density in the equation of standard curve to…
A: Protein contains aromatic residues which absorb the maximum light of 280 nm wavelength. The amount…
Q: For the following concentrations, determine the equivalent of a. 75ug/mg lyophilized protein in ppm…
A: Proteins are a type of macromolecule that have a variety of roles in biological processes. It is…
Q: Using the chart below, can you produce a two-step procedure that demonstrates protein purification…
A: Proteins are polymers of amino acids linked by peptide or amide bonds. Charge on the protein depends…
Q: Calculate the concentration of 0.2ml amino acid when path length is 1cm and has an absorbance value…
A: Beer-Lambert law states that amount of energy absorbed or transmitted by a solution is proportional…
Q: There is a mixture of four proteins with their respective MW (in kD) in parentheses: A (60) B (100)…
A: Chromatography is a method for separating a mixture in the laboratory. The liquid is immersed in a…
Q: We have isolated a mixture of 2 protein of the same molecular weight but different isoelectric…
A: The cationic means negative charge and anion is a positive charge column in column chromatography.
Q: An antibody binds to another protein with anequilibrium constant, K, of 5 × 109 M–1. When it binds…
A: The equilibrium constant, K, expresses the relationship between a reaction's products and reactants…
Q: A binding protein binds to a ligand L with a Kd of 400 nM. How much ligand is present when Y is (a)…
A: Kd is the dissociation constant. It is the equilibrium constant which indicates the dissociation of…
Q: Give possible biochemistry explanation why the American Heart Association recommend the use of…
A: As cardiovascular diseases are correlated with diets high in saturated fats, American Heart…
Q: Calculate the number of kcal and amount of protein provided by the following formula: 500 mL of 50%…
A: Energy is important for functioning of a living organism. It can be obtained from various sources…
Q: The cell membrane is both fluid and asymmetrical. Discuss asymmetry of the plasma membrane, as well…
A: The cell membrane is a thin, flexible, and selectively permeable layer that surrounds the cells of…
Q: Given this: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7…
A: The isoelectric point of a protein is the pH at which the net charge of the protein is zero. At this…
Q: 0.03 The uv spectrum of a protein solution shows A280 nm = 0.43 and A260 nm = What is the…
A: When working with proteins, one necessary part of any good assay is accurately determining how much…
Q: The kidneys function to eliminate ammonia from the blood. Based on ammonia’s pK value, what is the…
A: Kidney is a vital organ of the body that is involved in filtering out waste from the blood,…
Q: How does NH4SO4 affect water structure? What does this have to do with protein solubility? I thought…
A: One of the factors that determine the solubility of proteins in solution is the concentration of…
Q: Protein mixture contains four proteins alpl-4).blpl-6)l.clpl-9).dlpl+11). At pH-7, using cation…
A: Cation exchanger contain (-)ve charged stationary phase and (+)ve charged mobile phase. Hence,…
Q: Using the data in the table below, calculate the average molar mass of an amino acid residue in each…
A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…
Q: In a cell biochemistry study, 160,000 high-affinity binding sites for ADP were identified on each…
A: Given Values: [RT] = total binding sites on the platelets [R . L] = Bound sites with ADP [R L][RT] =…
Q: If crystals of deoxy Hb are exposed to air, they break. What does this imply about the 4o structure…
A: Hemoglobin (Hb) is an allosteric tetrameric protein that exists in equilibrium between the deoxy (T…
Q: Glutamic acid, Leucine, Arginine What is the ionization state at pH 10?
A: The pKa value of amino acid refers to the state of equilibrium between the deprotonated nitrogen and…
Q: Where would the following substances partition in water containing palmitic acid micelles? (a)…
A: Palmitic acid is a saturated long-chain fatty acid with a 16-carbon backbone. It is found naturally…
Q: Consider the following properties of the protein components of a sample mixture as provided in the…
A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…
Q: List 4 different actiated carrier molecules, for each one, list both the oxided and reduced forms
A: Cellular respiration is a metabolic pathway that breaks down glucose and produces ATP. The stages of…
Q: osmotic pressure
A:
Q: mg/L of cell tissue and 10 mg/L of ammonia
A: TKN is refers to the term Total Kjeldahl Nitrogen which is defined as the total concentration of…
Q: Why does the dissociation constant change for hemoglobin in high and low 02 concentrations? O…
A: The body needs energy. Energy comes from the breakdown of glucose. Oxygen is required in aerobic…
Q: In an experiment, separation of albumin from chicken liver was attempted at 31%, 58%, and 65%…
A: Ammonium sulphate is commonly used for protein precipitation, separation, and crystallization.…
Q: Given this: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7…
A: SDS PAGE is a denaturing PAGE where proteins are seperated based on mol weight in denaturing…
Q: You need to separate the following three proteins, Protein A (MW 76 600 Da; pI 4.5); Protein B (MW…
A: The chromatography techniques are laboratory techniques for the separation of a mixture into its…
Q: In the structure of vancomycin, what moiety contributes to its solubility in polar medium?
A: Vancomycin is a glycopeptide antibiotic and kills gram-positive bacteria such as…
Ovalbumin is one of the main proteins in egg white. If you consider a diameter of 5.4486 x 10-9 m and its specific volume of 0.75 cm3 / g.
a) Calculate the coefficient of diffusion of ovalbumin in water at 20 ° C
b) Determine its molecular weight.
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Estimate the TKN associated with a sample having 50 mg/L of cell tissue and 10 mg/L of ammonia. Assume cell tissue has a molecular composition of C5H7O2N.If a particular polypeptide that is very compact in 0.01 M NaCl expands considerably in 0.5 M NaCl, what forces are probably important in determining its overall size and shape?Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of the original suspension if the thousand-fold dilution of that suspension has an absorbance of 0.3 at 600nm? * A. 3.7 x 10^12 B. 3.7 x 10^9 C. 3.7 x 10^1 D. None of these is correct
- what is the dna concentration suspended in PO4 buffer at ph 7.0 that has an absorbance at 260 nm equal to 1.0?Student A says that he can insert any value of optical density in the equation of standard curve to determine the corresponding value of protein content. Does the student say it right or wrong? Explain why?The molecular formula for glucose is C6H12O6. What would be the molecular formula for a polymer made by linking ten glucose molecules together by dehydration reactions? Group of answer choices A. C60H120O60 B. C60H102O51 C. C60H100O50 D. C60H111O51 Use the following information to answer the following questions."The native structure of hemoglobin (HB) comprises of two α and two β subunits, each of which carries a heme group. There appear to be no previous studies that report the in-vitro folding and assembly of Hb from highly unfolded α and β globin in a 'one-pot' reaction. One difficulty that has to be overcome for studies of this kind is the tendency of Hb to aggregate during refolding. This work demonstrates that denaturation of Hb in 40% acetonitrile at pH 10.0 is reversible." (J Am Soc Mass Spectrum 2007, 18, 8-16)In sickle-cell disease, as a result of a single amino acid change, the mutant hemoglobin tetramers associate with each other and assemble into large fibers.…
- If a protein lysate obtained is 1003 ug/ml What would be the total concentration of each lysate that is loaded into their well if you load just 25 ul of each?Most protein dissolve in neutral salt solution, is that true? Provide reasoningHow many milliliters of 0.0050 N KOH are required to neutralize 25 mL of 0.0050 N H2SO4? To neutralize 25 mL of 0.0050 M H2SO4?
- A mixture of proteins contains four different polypeptides, all in ~equal concentration, in solution with the following properties: Protein Molecular Mass (kDa) Isoelectric point A 45 4.5 B 77 6.0 C 28 4.1 D 14 10.7 A fraction of the protein solution is applied to a strong cation exchange column using a buffer at pH 8.0 with increasing [NaCl] from 0.05 M – 1.0 M. The chromatogram is shown below: 1. Based on the data presented, which of the following statements is true: Peak #1 is protein D Peak #4 is protein C Peak #3 is protein A Peak #4 is protein D Peak #2 is protein B 2. Since you know that the proteins are all present in approximately equal concentrations, the different relative peak areas tell you that: There are more neutral amino acids in protein #4…In a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are: HC2H3O2 < -----------> H+ + C2H3O2-If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?