If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 6.7P
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If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
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