If an antibody binds its antigen with affinity of 150 nanomolar, what is the standard binding energy for this antibody-antigen pair? Assume 25°C, pH 7 throughout, and biochemical convention; choose closest answer. A1. -40 kJ / mol A2. -20 kJ / mol A3. 0 kJ / mol A4. +20 kJ / mol A5. +40 kJ/mol
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- In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the amount of cholesterol in the serum sample is determined as 3.11 g/L, what is the absorbance value of the sample? A. 0,280 B. 0.028 C. 0.208 D. 0.82KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?
- Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of the original suspension if the thousand-fold dilution of that suspension has an absorbance of 0.3 at 600nm? * A. 3.7 x 10^12 B. 3.7 x 10^9 C. 3.7 x 10^1 D. None of these is correctFor numbers A to I, determine the type of solubility each drug has based on USP: Freelysoluble Soluble Sparinglysoluble Slightlysoluble A. 1ml of water is needed to dissolve 1 gram of procaine B. 32ml of water is needed to dissolve 2 grams of morphine C. 1liter of water is needed to dissolve 5 grams of D. 150ml of water is needed to dissolve 5 grams of codeine sulfate E. 1liter of water is needed to dissolve 1 gram of phenobarbital F. 120 ml of water is needed to dissolve 1 gramof G. 1gram of silver sulfadiazine dissolves in 2 ml of H. 10grams of phenobarbital sodium can dissolve in 10 ml of I. 1gram of morphine sulfate requires 16 ml of water to beGive typing answer with explanation and conclusion Consider the following pharmacokinetic information: Desired AUC:MIC 400 mg/L x hr Vancomycin CL 2.5L/hr What dose would be required to have the desired AUC:MIC for a given patient?
- Using conversion factors, solve each of the following clinical problems:a. The physician has ordered 1.0 g of tetracycline to be given every six hours to a patient. If your stock on hand is 500-mg tablets, how many will you need for one day’s treatment?b. An intramuscular medication is given at 5.00 mg / kg of body weight. What is the dose for a 180-lb patient?c. A physician has ordered 0.50 mg of atropine, intramuscularly. If atropine were available as 0.10 mg /mL of solution, how many milliliters would you need to give?d. During surgery, a patient receives 5.0 pt of plasma. How many milliliters of plasma were given?Using conversion factors, solve each of the following clinicalproblems:a. A nurse practitioner prepares an injection of promethazine,an antihistamine used to treat allergic rhinitis. If thestock bottle is labeled 25 mg /mL and the order is a doseof 12.5 mg, how many milliliters will the nurse draw up inthe syringe?b. You are to give ampicillin 25 mg/kg to a child with a massof 62 lb. If stock on hand is 250 mg /capsule, how manycapsules should be given?After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.
- What is measured at the A280 absorbance values?Use correct sig figs The concentration of a purified monoclonal antibody was measured using UV280 nm. The sample was diluted (200 μL of purified antibody in 800 μL buffer) prior to analysis using a spectrophotometer. Calculate the concentration of antibody in the purified fraction if the Abs=0.95 of the diluted antibody. The molar absorptivity is known to be 191,411.6 M-1cm-1 and the molecular weight is 150 kDa. The pathlength for the cuvette is 1 cm.Calculations Involving Units, mcg/mg and Other Measurement of Potency. Show your complete solution. 1. A hospital pharmacy has available 2-mL prefilled syringes containing 80 mg of tobramycinand 1.5-mL prefilled syringes containing 60 mg of tobramycin. The syringes are calibratedin 0.25-mL units. Explain how you would prepare a medication order calling for 110 mgof tobramycin to be added to 100 mL of D5W for intravenous infusion.
