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- Could someone explain what this is saying? I would like a better understanding. FIGURE D CPP32 (Caspase-3) protease activity. Aliquots of cell lysates (50μL) were incubated with an equal volume (50 μL) of the reactionbuffer and 5 μL of 7-amino-4-trifluoromethyl coumarin (AFCDEVD) for 1 hour at 37˚C (Clontech). The shift in fluorescenceemission of AFC-DEVD, on its proteolysis to free AFC by theprotease, was detected in a fluorometer, using a 400 nm excitationfilter and 505 nm emission filter.CaCO3(s)<->Ca^2+(aq)+CO3^2-(g). write down Ksp for this reaction1. The slope of a standard curve of Abs vs [protein] is equalivent to which Lambert-Beer variable? A.) OD B.) ϵ C.) c D.) D 2.) DNA absorbs light around 260 nm. What wavelength do proteins absorb? A.) 625 nm B.) 140 nm C.) 240 nm D.) 280 nm