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Do you think a patient needs to have the SAME two mutant alleles of CFTR to present with/have cystic fibrosis? Why or why not?
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- cggcaggagc cccccgacct cccaggcgga ccgccctccc tccccgcgcg cgggttccgg gcccggcgag agggcgcgag cacagccgag gccatggagg tgacggcgga ccagccgcgctgggtgagcc accaccaccc cgccgtgctc aacgggcagc acccggacac gggcctcagcc actcctacat ggacgcggcg cagtacccgc tgccggagga ggtggatgtg ctttttaaca tcgacggtca aggcaaccac gtcccgccct actacggaaa ctcggtcagg Provide the primers for the nucleotide sequence above (GATA3)geneSspI --- 5' AAT - ATT 3'5' GGATAATATT GTTAACAATCTCTACGGGTTAACACCCTTGGAATATTTTAA 3' 3' CCTATTATAACAATTGTTAGAGATGCCCAATTGTGGGAACCTTATAAAATT 5' Number of pieces of DNA _______TGTCTAGTTGGCAATTGTCCTAGCCATACTGT
- Step1:Clickwhereitsays“StartaNewGame.”Inordertomakenewbodycells,thecellcycle mustoccur.Duringthecellcycle,onecelldividestoformtwodaughtercellswithexactlythe same___________.Thecellcycleincludesthefollowing:________________________________, _________________________,and_________________________.Interphasehappens__________ ______________.DuringInterphase,thecelldoesnormalactivities,likemakingproteins,for example.HindII --- 5' GTC - GAC 3', HaeIII --- 5' CC - GG 3', EcoRI --- 5' G - AATTC 3' and BamI --- 5' CCTAG - G 3' 5' AGAATTCTTACGCCGGACGTACCTAGGTTTAGTCGACTC CGCCGCCCCTAGGGTCATCA 3' 3' TCTTAAGAATGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT 5' Number of pieces of DNA , and blunt end fragment (s), and sticky end fragment(s)MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH MVH ltp eeksavta lwgkvnvdevggealg rllv vypwtqrffesfg dlstp davmgnpkvkahgkkvlgafsdglahl dnlkgtf atlselhcdkhvdpenfrllgnvlcvlahhfgkef tppvqaayqkvvagvanalahkyh MVH ltp eeksavta lwgkvnvdevggealg rllv vypwtqrffesfg dlstp davmgnpkvkahgkkvlgafsdglahl dnlkgtf atlselhcdkhvdpenfrllgnvlcvlahhfgkef tppvqaayqkvvagvanalahkyh…