Part II Data Analytical Wavelength read from the spectra (nm): Absorbance at the analytical wavelength for the 5 mL/5 ml mix Absorbance at the analytical wavelength for the 4 mL/6 ml mix 628.5 0.875 0.704 3. Find the molar extinction coefficient from the 4 mL/6 ml mix. Pathlength: 1.00 cm Volume of Brilliant Blue: 4.00 mL Volume of DI water 6.00 ml Absorbance at the analytical wavelength for the 4 mL/6 ml mix: 0.704 Concentration of Brilliant Blue in the 4 mL/6 ml. mix (M): Molar extinction coefficient from the 4 ml/6 ml mix (M'cm') 4. Average molar extinction coetficient for Brilliant Blue (M 'cm)

Part II Data Analytical Wavelength read from the spectra (nm): Absorbance at the analytical wavelength for the 5 mL/5 ml mix Absorbance at the analytical wavelength for the 4 mL/6 ml mix 628.5 0.875 0.704 3. Find the molar extinction coefficient from the 4 mL/6 ml mix. Pathlength: 1.00 cm Volume of Brilliant Blue: 4.00 mL Volume of DI water 6.00 ml Absorbance at the analytical wavelength for the 4 mL/6 ml mix: 0.704 Concentration of Brilliant Blue in the 4 mL/6 ml. mix (M): Molar extinction coefficient from the 4 ml/6 ml mix (M'cm') 4. Average molar extinction coetficient for Brilliant Blue (M 'cm)

Chemistry & Chemical Reactivity

10th Edition

ISBN:9781337399074

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions

Section: Chapter Questions

Problem 77PS

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Record your absorbance data for the YELLOW and RED dyes below. Calculate the dye concentration in mM for each cuvette using M1V2 = M2v2 . Remember the concentration of stock solutions (100^ % dye) is 0.020 mM(mM= millimolar =mm /L). Data for YELLOW: peak absorbance used on spectrophotometer =420nm Cuvette 1 - 100% dye = 0.680 absorption Cuvette 2 - 75% dye = 0.510 absorption Cuvette 3 - 50% dye = 0.340 absorption Cuvette 4 - 25% dye = 0.170 absorption Data for RED - peak absorption used on spectrophotometer = 500nm Cuvette 1 - 100% dye: 0.621 absorption Cuvette 2 - 75%: 0.466 absorption Cuvette 3 - 50% dye: 0.311 absorption Cuvette 4 - 25% dye: 0.1555 absorption
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The absorbance for a dye sample of unknown concentration is measured and the corrected absorbance is 0.666. The standard curve created is found to have this linear equation: y = 0.153x + 0.043 Calculate the concentration (ug / mL) of the dye sample. 4.54 3.33 4.07 5.17 2.07
Do read carefully and answer accordingly Chlorophyll a (a plant pigment) has the following molar extinction coefficient: εM662 = 8.4 x104 M-1 cm-1 A solution of different plant pigments has an absorbance of 0.632 at 662nm. Calculate the concentration of chlorophyll a in your sample assuming a light path length of 1 cm. -1.3 x 105 M -9.5 x 10-4 M -7.5 x 10-6 M -1.3 x 106 M
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A solution prepared by dissolving 25.8 mg of benzene (C6H6, FM 78.11) in hexane and diluting to 250.0 mL has an absorption peak at 256 nm, with an absorbance of 0.266 in a 1.000-cm cell. Hexane does not absorb at 256 nm. Find the molar absorptivity of benzene at this wavelength.
The absorption coefficient of rhodopsin at 496 nm is 40,000 M-1cm-1. If you have a 1M solution of rhodopsin, how many times will you dilute the solution so that the diluted solution will give you an absorption reading at 496 nm that is in the range of 0.1-1.0. And what will be the absorption by the diluted solution?
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