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PCR can be utilized _______
| a. |
as a cloning vector |
|
| b. |
to modify a human genome |
|
| c. |
all of the choices |
|
| d. |
in DNA profiling |
Step by step
Solved in 2 steps
- PCR can be used______ . a. as a cloning vector b. in DNA profiling c. to modify a human genomeAll are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚CDefinition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organism
- The PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase (d) restriction enzymes (e) b and cA) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
- Forensics a. The importance of recombinant DNA technology in Forensics b. Potential products produced and processes involved in forensics (at least 5 examples in each field). c. The downside/ disadvantages of recombinant DNA technology in forensicsWhich process consists of modifying an individual’s genome? a. DNA profiling b. SNPing c. genetic engineering d. DNA hybridization e. PCRScientists can distinguish between DNA of different individuals, thus making this information useful in criminal investigations. The technique used is called __________. a. DNA fingerprinting b. CRISPR/Cas9 c. fluorescent in situ hybridization d. PCR
- PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genomeDefinition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified OrganismWhat would be the outcome if the primers used in a polymerase chain reaction have lower GC content (<40 %), shorter, and more variable than the intended oligonucleotide sequence? a.The PCR reaction will cease after the first cycle b.The reaction will yield a mixture of non-specific products. c.All of these d.The PCR reaction will not start. e.The reaction will yield a single short PCR product.
