During the plasmid extraction, your lab partner added solution 2 and vigorously vortexed the tube. How will this impact your results? a. The final tube will contain plasmid DNA, RNA, and bits of genomic DNA. b. It will work better because everything will be mixed well. O c. The final tube will not contain any DNA. d. None of the above.

During the plasmid extraction, your lab partner added solution 2 and vigorously vortexed the tube. How will this impact your results? a. The final tube will contain plasmid DNA, RNA, and bits of genomic DNA. b. It will work better because everything will be mixed well. O c. The final tube will not contain any DNA. d. None of the above.

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 4RQ: What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel...

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What happens if you forget to add Sybrsafe to a gel that will be used for DNA gel electrophoresis a. there will only be bands in your sample lanes b.neither the ladder or your sample lanes will have bands c.your ladder lane will be the only one contanining bands d.the gel will run normally and bands will show up for both the ladder lane and the sample lanes
DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.
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Choose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmid
A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion? B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be? C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?
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What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reaction
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You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.