Restriction enzymes found in bacterial cells are ordinarily useda. during DNA replication.b. to degrade the bacterial cell’s DNA.c. to degrade viral DNA that enters the cell.d. to attach pieces of DNA together.

Q: In an EMSA, the binding of a protein to DNAa. prevents the DNA from being digested with a…

A: Electrophoretic mobility shift assay (EMSA) is used to identify deoxyribonucleic acid (DNA)-binding…

Q: Compare the process of polymerase chain reaction (PCR) to DNA replication.  Discuss three…

A: PCR is in vitro process whereas DNA replication is in vivo process.

Q: ase enzymes are used in recombinant dna technology

A: Restriction enzymes (also called restriction endonucleases) cleave double-stranded DNA at very…

Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…

A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…

Q: PCR has many useful applications. However, an incorrect application of PCR is: a) Amplification of…

A: DNA or Deoxyribonucleic acid is a macromolecule which is responsible for the transfer of hereditary…

Q: Enzymes used to cut genes in recombinant DNA research are known as ____.   a. DNA polymerases   b.…

A: Enzymes  Protein molecules that act as catalyst in a biological processe or reaction.

Q: Which of these is a polymer made of nucleotides?  a. RNA polymerase  b. CAS9 c. restriction…

A: A nucleotide has three parts: a phosphate group, a 5-carbon sugar, and a nitrogenous base. They are…

Q: Restriction enzymes (type II) bind to their recognition site and A. begin elongation from it B.…

A: Restriction endonucleases are those enzymes synthesized from bacterial cells that play an important…

Q: Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium…

A: Deoxyribonucleic acid (DNA) is a particle made out of two polynucleotide chains that loop around one…

Q: Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the…

A: Vast uses of endonuclease activity of restriction enzyme found in the biotechnology for gene…

Q: Describe restriction enzymes and explain why their discovery wasimportant for DNA research.

A: BASIC INFORMATION ABOUT ENZYMES They are the catalyst. They help in accelerating the chemical…

Q: Explain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain…

A: Recombinant DNA technology is the combining of the DNA molecules from two different species. The…

Q: In nature, the purpose of restriction enzymes is to O A. Protect the bacterium from virus attack by…

A: Restriction Enzymes Restriction enzymes (endonucleases) cut DNA at specific sequences. They broken…

Q: Shotgun sequencing is a method of DNA sequencing in whicha. the DNA fragments to be sequenced are…

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: in Cohen-Boyer's recombinant DNA procedure _______ must be used for both the bacterial DNA and the…

A: Introduction Cohen and Boyer made recombinant DNA molecules by using inserting a gene of interest…

Q: DNA fragments cut by restriction enzymes can form two types of ends. What are these ends called? •…

A:

Q: Which technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: In 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to…

A: Researchers continue to find new medications that aid in the treatment of deadly diseases and enable…

Q: A protein that can cut DNA at specific DNA base sequences is called aa. DNase. c. restriction…

A: A cell is the basic building block of life. It contains the genetic material of each organism in its…

Q: We have mRNA prepared from human cells but PCR needs DNA. What should we do?

A: A polymerase chain reaction (PCR) is a technique that is used in molecular biology to amplify a…

Q: Features of restriction enzymes include the following, except  __________ . a. They are produced…

A: Restriction enzyme is a group of enzymes which involves the cleaving of DNA molecule at a specific…

Q: Modern researchers can manipulate genetic material to alter genes and blend plant, animal and…

A: Genetic engineering: Also called genetic modification, it is defined as the process in which the DNA…

Q: What is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific…

A: Nucleic acids are large molecules that are made up of nucleotides that performs a various function…

Q: E) double stranded DNA separates into single stranded DNA

A: The correct option is E . Double stranded DNA separates into Single stranded DNA .

Q: Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on…

A: Genes are the structural and functional units of heredity that carry coded genetic information in…

Q: A DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the…

A: DNA fingerprinting is commonly employed in investigations to connect evidence with suspects, but DNA…

Q: Genes of Interest can be selected from a genomic library by using (a) cloning vectors o) DNA probes…

A: Vector refers to a DNA molecule which is used as a vehicle to carry genetic material from one cell…

Q: The restriction enzymes used in gene-cloning experiments, which generates sticky ends that can .a.…

A: Deoxyribonucleic acid (DNA) is a long molecule. DNA contains the instructions an organism required…

Q: Cellular DNA replication uses the enzymes for the process while PCR uses thermal denaturation that…

A: DNA replication is the biological process of delivering two indistinguishable imitations of DNA from…

Q: Why there is no detectable fluorescence in the samples after the first few PCR cycles? O A. Because…

A: To multiply the target DNA, the PCR processes of denaturation, heating, and elongation are cycled…

Q: X-ray Irradiation is   A. Chemical mutagen   B. Physical mutagen   C. Cosmetics agent   D.…

A: Mutagens are chemical compounds or forms of radiation (such as ultraviolet (UV) light or X-rays)…

Q: Choose the combination of answers that most accurately completes the statement. Which of the…

A: Gene cloning is the process of employing genetic engineering techniques to create perfect clones of…

Q: Illustrate the steps in restriction digestion and PCR

A: Polymerase chain reactions is a technique used to make millions of copies rapidly of a DNA sample.…

Q: Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a.…

A: DNA is an important biomolecule present in the cell as it is responsible for various traits…

Q: The activity of restriction enzymes may produce fragments with sticky ends.  Sticky ends are   a) a…

A: Correct option is (c). Restriction enzyme, also called as restrictions endonucleases,  is a…

Q: The PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase…

A: The polymerase chain reaction, or PCR, is a chemical reaction used by molecular biologists to…

Q: Which step is NOT included in the technique called Southern blotting? O a. visualization by…

A: Southern blotting is a laboratory procedure that is used to detect a specific DNA sequence in a…

Q: Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA…

A: Restriction enzymes are restriction endonuclease that recognizes particular restriction sites on the…

Q: Make a table comparing the advantages and disadvantages of PCR and gene cloning for amplifying DNA…

A: PCR or polymerase chain reaction is a method by which small fragments of DNA are used as a template…

Q: Which of the following is true of reverse transcriptase?a. It is required for the movement of DNA…

A: The enzyme engaged in reverse transcription is reverse transcriptase. The enzyme works through using…

Q: PCR can be used______ . a. as a cloning vector b. in DNA profiling c. to modify a human genome

A: DNA is the genetic material present in the cells of living beings.

Q: Following are four processes common to most cloning experiments:  a)    transforming bacteria b)…

A: A "clone" simply means that an object, cell, or whole organism has the same genetic makeup as the…

Q: Which of the following tools of DNA technology is incorrectlypaired with its use?(A)…

A: As in the recent times, the approach towards genetics has gone up a high level. Various techniques…

Q: In the "DNA Extraction from Fruit" laboratory, DNA from a banana, kiwi or strawberry was extracted.…

A: DNA Or deoxyribonucleic acid is the common biomolecule present in living things, that is responsible…

Q: separates molecules by movement due to size and electrical charge              [ Choose ]…

A: Macromolecules and their fragments can be separated by their movement due to size and electrical…

Q: which method in biotechnology is used to read and understand an oganisum DNA

A: Introduction: a method primarily used to alter the phenotypic of an organism (host) after…

Q: PCR is essential for Select one: A. allowing restriction enzymes to cut DNA. B. making many copies…

A: Polymerase chain reaction (PCR) is a typical research facility method used to make many duplicates…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.