PCR tests are becoming more prevalent as a standard for testing for bacterial or viral disease. Describe how PCR would be used in place of a growth culture for Strep throat and why this would be advantageous.
Q: How are genetic forensics inaccurate? Provide source for proof
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Q: What method is used to transfer plasmid DNA into bacteria? Group of answer choices heat shock…
A: Plasmid It is an extra chromosomal piece of DNA found is many of the prokaryotes.
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A: The answer is False. Primers are small, chemically synthesised oligonucleotides that are…
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Q: Pcr based paternity test
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A: Polymerase Chain Reaction (PCR) is a molecular biology technique for duplicating a DNA sequence.…
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Q: Describe the applications of PCR in identifi cation techniques.
A: The polymerase chain reaction is based on the utilization of the polymerase enzyme. It is studied…
Q: Explain how PCR can be used to detect infectious agents in diagnoses of diseases.
A: PCR stands for Polymerase Chain Reaction. Using this technique, a fragment of DNA can be amplified.…
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A: DNA fingerprinting is a chemical test that shows the genetic makeup of a person or other living…
Q: Identify if the following statements are true or false. Quantitative PCR is also known as the…
A: A real-time polymerase chain reaction is a molecular genetics lab technology that involves the…
Q: What is the purpose of the first step of PCR when it increases the temperature to 95 C.
A: The first step of PCR is to melt the DNA so that double-stranded DNA separates into single-stranded…
Q: PCR is a useful tool for early diagnosis of an infectious disease.Elaborate.
A: A disease refers to an abnormal condition in the body that have negative impact on the function or…
Q: Describe how PCR can be used to diagnose diseases.
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Q: Write 3 paragraphs, 3-5 sentences long, on how this technique is used in an industrial,…
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A: PCR stands for polymerase chain reaction. It is used to synthesize the specific region of DNA.
Q: What is the addition and limitation of the RT-PCR approach? Keep CoViD-19 in mind
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Q: Place the stages of PCR in the correct order -Anneal -Denature -Analyse -Extend
A: Polymerase chain reaction (PCR) is a laboratory technique for rapidly amplifying DNA to produce…
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Q: Briefly explain Polymerase Chain Reaction (PCR) applications in clinical diagnosis.
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A: what is DNA profiling DNA profiling (also known as DNA fingerprinting) is the process of determining…
Q: How PCR may be used for the detection of disease–causing pathogens in a population during COVID…
A: In PCR, the infected gene is amplified and allows them to bind with the complimentry strand. After…
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A: If the collected DNA at the crime scene is from the suspect but don’t have enough to run a test to…
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A: Thank you for the question Answer = A consists of a selectable marker, reporter gene and…
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A: There are certain equipments that must be present in a biotechnology laboratory: Centrifuge : It…
Q: Which of the given statements is correct in the context of visualizing DNA molecules separated by…
A: In Agarose Gel electrophoresis DNA fragments are separated under size. The negatively charged DNA…
Q: blue-white colony selection method in the recombinant colony screening
A: Blue - white screening for selection recombinat colony :- Blue - white screening is a rapid and…
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A: PCR is Polymerase Chain Reaction used for the amplification of given DNA molecule.
Q: Define the advantage of using PCR for infectious disease diagnosis.
A: Polymerase Chain Reaction is referred to as PCR. The process of amplifying DNA sequences and…
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A: The use of spin columns for RNA isolation is a common practice in molecular biology laboratories.…
Q: Explain why PCR is useful for infectious disease diagnosis.
A: Polymerase chain reaction or PCR is a technique used for the amplification of a small portion of the…
Q: Which of the following are the two methods of screening?a) Hybridization and PCRb) ELISA and…
A: BASIC INFORMATION SCREENING It is a biological process which is used to identify any specific set…
Q: use PCR to assess microbial activity, what type of PCR would you use and why?
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Q: les of heat, primers and the bacterium Thermus Aquaticus in the process of PCR.
A: The polymerase chain reaction (PCR) is a common molecular biology technique that allows researchers…
Q: Describe the three main steps of Polymerase Chain Reaction (PCR)
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Q: OUTLINE WHAT CAN BE USED AS A POSITIVE OR NEGATIVE CONTROL IN YOUR TEST IF YOU PERFORM PCR TARGET…
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- Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR). Which of the tests had the highest accuracy and what methodology and techniques is used for Multiplex PCR test.Explain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.
- Which of the following methods can be applied to detect the infectious virus Immuno-electron microscopy Plaque assay Western blot Hemagglutination inhibition Quantitative PCRPCR can be used to identify an unknown bacterium because ?Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
- Electro blotting techniques are routinely used in diagnostic microbiology for detection of a specific DNA sequence in the given bacterial culture. Explain.THE SPECIATION OF THE TWO MOST COMMON FUSOBACTERIUM PATHOGENS CAN BE ACCOMPLISHED BY WHICH BIOCHEMICAL TESTWhat is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply A. To observe colony morphology/color B. To isolate viral plaques C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,) D. To isolate a clonal population of bacteria from the rest of the culture E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly) F. Accurate quantification (to calculate CFU/mL) G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate. H. To lower risk of contamination/identify contamination
- you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)A patient comes in with an infection and lab analysis show that the infectious agent is Staphylococcus aureus. Doctor prescribed an antibiotic and told the patient that the infection should clear in 10 days. After 10 days patient comes in with the situation is worsening. Doctor is suspicious of a resistant strain and wants to start another culture to identify the stain and the mutation causing the resistance. What type of media you would use to grow the bacteria sampled from the patient?You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.