DNA can be electrophoresed. all cells have RNA. DNA polymerase will replicate any bacterial DNA. all cells have DNA. the DNA primer is specific to particular target DNA sequence
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- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.In a PCR reaction, a few seconds at high temperature disrupts the ____ that hold the two strands of DNA together, so every molecule of DNA unwinds and becomes single-stranded. DNA ligases covalent bonds peptide bonds hydrogen bonds lipoproteinsAssuming you are working with the DNA from a single organism, in DNA gel electrophoresis the different bands in the final gel result because the DNA molecules ________. are from different organisms have different lengths have different nucleotide compositions have different genes
- Which of the following enzyme is required for end to end joining of DNA?a) DNA ligaseb) Restriction endonucleasec) RNA polymerased) DNA polymeraseWhy is the following statement fals? RNA polymerase is a dangerous enzyme to the cell because it does not proofread as well as DNA polymeraseWhich of the following statements about gel electrophoresis is false? Choose all that apply. Smaller DNA fragments travel faster and further during gel electrophoresis. DNA fragments can be separated based on fragment length. DNA fragments can be separated based on DNA sequence. Larger DNA fragments travel faster and further during gel electrophoresis.
- What special property does Taq polymerase have? It binds to each strand of DNA after the two strands of DNA have separated. It is highly specific for bacterial DNA. It can tolerate a higher temperature. It allows the DNA double helix to open. It requires less ATP to function than other polymerases.Which of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.Fill in the blank 1. A genetically engineered rodent in which a normal gene has been replaced by a a defective version of the same gene is called ______. 2. DNA fingerprints (ex: in forensics) can be generated by selectively cutting DNA with bacterially derived proteins called _______ and examining the resulting DNA fragment patterns on agarose gels.
- What goes into the reaction tube for Sanger sequencing? Template DNA, primers, DNA polymerase, and fluorescent dNTPs. Template DNA, primers, DNA polymerase, dNTPs, and fluorescent ddNTPs. Primers, dNTPs, and fluorescent ddNTPs.Which of the following cuts DNA like molecular scissors? Clustered regularly interpaced short palindromic repeats Cas (CRISPR associated ) proteinsWhich of the following statements is false?a) BAC is a circular DNA moleculeb) YAC is a linear DNA moleculec) Taql is a DNA polymerased) BAC is a linear DNA molecule
