pH 3.5 4.5 5.5 6.5 7.5 8.5 Dilution Factor 5 5 5 50 50 50 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Calculate the amount (in grams) of soluble protein in the soy flour extract **Make sure to account for the dilution you made in Step 1 (Assume the volume of acid or base used to adjust the pH is negligible). Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml)
step 1: Prepare a 1:15 soy flour-water mixture by adding l g soy flour to 15 ml distilled water in a 50-ml Falcon tube and vortex it to form a uniform suspension;
|
pH |
3.5 |
4.5 |
5.5 |
6.5 |
7.5 |
8.5 |
|
Dilution Factor |
5 |
5 |
5 |
50 |
50 |
50 |
|
Absorbance |
0.098 |
0.027 |
0.068 |
0.028 |
0.032 |
0.054 |
|
Concentration in diluted supernatant (mg/ml) |
0.196 |
0.054 |
0.136 |
0.0056 |
0.0064 |
0.0108 |
|
Concentration in undiluted supernatant (mg/ml) |
0.98 |
0.27 |
0.68 |
0.28 |
0.32 |
0.54 |
Calculate the amount (in grams) of soluble protein in the soy flour extract
**Make sure to account for the dilution you made in Step 1 (Assume the volume of acid or base used to adjust the pH is negligible).
Soluble protein (g) = (C x V x F) / 1000
C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml)
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