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- How does the compound microscope achieve magnification? resolution? Cite your reference/s if possible.The magnification possible with any type of microscope is an important property, but its resolution, the ability to distinguish between two very closely apposed objects, is even more critical. Describe why the resolving power of a microscope is more important for seeing finer details than its magnification.In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.
- Why don’t most light microscopes use 303 ocular lenses for greater magnification?Cite the advantages and disadvantages of the two compared microscopesa. Bright-Field vs Dark-Field Microscopeb. Phase-contrast vs Fluorescence Microscopec. TEM vs SEMWhat is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-Probe
- What major advantage does phase-contrast microscopy haveover staining?Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?A microscope that maintains focus when the objective magnification is increased is calleda. binocular.b. myopic.c. parfocal.d. refractive.e. resolute.