Asked Oct 1, 2019

Please answer these two questions regarding PCR:


a) Why do you need to perform PCR on DNA obtained from a crime scene?

b) Why so forensic labs analyze non-coding DNA rather than genes? 


Expert Answer

1 Rating
Step 1

The polymerase chain reaction (PCR) is a technique used to make many copies of a specific segment of DNA. The key components of polymerase chain reaction include Taq polymerase, template DNA, primers, nucleotides, and buffer. The three main steps of PCR are, denaturation, annealing, and extension.

Step 2

a) PCR technique is used to amplify DNA approximately a billion times within 35-40 cycles. DNA collection and analysis is an integral part of crime scene analysis, and the samples obtained are often extremely low in concentration and low quality. PCR is usually used to amplify the DNA to amounts that...

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