What are the DNA extraction methods? Explain each one.

Q: What are STRs, and why are they useful for DNA profiling?

A: Introduction  In the genome there are usually two type of nucleic acid sequence present. One is Non…

Q: Is the DNA isolated from Cheek Cells using Saline solution, Detergent, and Ice-cold alcohol pure or…

A: DNA is a genetic material present in every cell.

Q: Why is MgCl2 added to the PCR reaction?

A: De-novo gene synthesis is becoming an important tool for recombinant DNA technology. The Denovo gene…

Q: What is the mom method of pacaking DNA?

A: Answer: DNA : Deoxyribonuleic Acid acts as the genetic material which transfers the genetic…

Q: Which is the correct order of the PCR reaction?

A: Introduction PCR or the polymerase Chain Reaction is a molecular technique used to amplify a…

Q: How does magnetic bead DNA extraction work?

A: DNA extraction is the method of isolating DNA by disrupting the cell wall and nuclear membrane with…

Q: What is real-time PCR?

A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…

Q: What does the alcohol do in this experiment? Why does the DNA rise to the top after adding alcohol?…

A: Extraction of DNA from fruits.

Q: What are the benefits of using a mixture classification scheme as outlined in DNA Box 14.1? What…

A: INTRODUCTION German stain commission This commission includes several forensic science and legal…

Q: What is the function of the homogenizing solution in DNA isolation? What are its components?

A: In the process of DNA isolation it is very important to breakdown or lyse the cell this allow the…

Q: Why is the annealing temperature different between samples during DNA extraction & marker gene…

A: Introduction Polymerase chain reaction or PCR  laboratort technique by which millions to billions of…

Q: What is PCR bias? Why is it undesirable?

A: Answer: Introduction: Polymerase chain reaction (PCR) is a process extensively used to quickly form…

Q: What is PCR amplification?

A: In the field of molecular biology, however, cloning is viewed at a genetic molecular level, where a…

Q: What is cell lysis? Discuss the principles behind DNA isolation (extraction and precipitation…

A: A eukaryotic cell consists of many membrane-bound organelles. The membrane-bound organelles form…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: DNA is a hereditary molecule found in cells that transmits genetic information from one generation…

Q: Why is extra primers added to the pcr reaction?

A: Introduction :- The polymerase chain reaction, or PCR, is a chemical reaction used by molecular…

Q: What is linear amplification PCR?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: Name four reagents used during a PCR reaction

A: Polymerase chain reaction: It is an in vitro technique that results in the exponential…

Q: Why were both “+ DNA” and “- DNA” mixtures prepared and plated?

A: Both “+DNA” and “-DNA” mixtures prepared and plated because in the transformation protocol “+DNA”…

Q: what is the Jusage of DNA separation in gel electrophoresis

A: DNA is abbreviated as deoxyribonucleic acid, which is known as hereditary material in most organisms…

Q: What is the purpose of adding the dishwashing liquid to the DNA extraction?

A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: Sanger sequencing method is chain terminating method.

Q: Describe two methods in performing a DNA analysis, its possibleapplications, and how it can be so…

A: DNA (Deoxyribonucleic acid) analysis is a method of determining the characteristics of the…

Q: Is it possible to obtain DNA in cloverleaf configuration? Explain

A: Cloverleaf Model:  The cloverleaf model of tRNA is a representation of the molecule's structural…

Q: How is DNA fragmentation test done?

A: Deoxyribonucleic acid (DNA) is the main constituent of the chromosome. It contains all information…

Q: Why is it important to wear gloves when setting up the PCR tubes?

A: PCR represents polymerase chain reaction which is an in vitro laboratory technique used for the…

Q: Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA…

A: A protein fragment that is obtained when the DNA polymerase I of E.coli is enzymatically cleaved…

Q: what is   Inverse PCR ?

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: What are some difficulties with low copy number DNA analysis? What kinds of checks and balances can…

A: INTRODUCTION DNA analysis DNA analysis is a method of analyzing the characteristics of DNA. It is…

Q: What are the principles of Chelex extraction and solid-phase DNA extraction methods?

A: To explain the principles of Chelex extraction and solid-phase DNA extraction methods The principle…

Q: Is the DNA soluble in the aqueous solution or alcohol?

A: Solubility of the substance depends on molecular polarity. Polar molecules are hydrophilic…

Q: What is DNA microarray used for?

A: It is a laboratory tool used to detect the expression of thousands of genes at the same time. The…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…

Q: When is the transformation factor used?

A: According to Griffith's experiments, based on the bacterial transformation through the Streptococcus…

Q: What is a PCR?

A: PCR is a technique widely used in molecular biology and it was developed by Kary Mullis in 1983.

Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer?

A: The deoxyribonucleic acid (DNA) carries the genetic information and is usually present in the…

Q: What is a Sanger Sequencing ?

A: The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide…

Q: What are the reagents and equipment used in PCR?

A: Polymerase Chain Reaction(PCR) is a laboratory technique introduced by Kary Mullis in 1980s to…

Q: How to identify the type of genomic DNA isolation contamination and how to eliminate this…

A: It is chromosomal DNA, in contrast to extra chromosomal DNAs like plasmids. Most organisms have the…

Q: Why is the DNA stored cold with the InstaGene matrix after boiling the samples?

A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…

Q: What is the purpose of the PCR?

A: PCR is a technique which is used for the amplification of specific segments of DNA from a larger DNA…

Q: Why is a primer needed at each end of the DNA segment beingamplified by PCR?

A: Primer - Primer is a kind of short nucleotide sequence which is used as a starting point in the…

Q: What types of DNA can be extracted? Explain your answer.

A: Some of the most common DNA extraction methods include organic extraction, Chelelx Extraction and…

Q: Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the…

A: The correct option is b 5'CCATTGG3'. Sanger sequencing ( method of DNA sequencing and it involves…

Q: What part of the PCI Method of DNA extraction explains the: Cell Lysis Separation Precipitation

A: PCI DNA extraction method uses phenol, chloroform, and isoamyl alcohol as three basic ingredients to…

Q: What is DNA phenotyping, and how do law enforcement agencies use this profiling method?

A: DNA (deoxyribonucleic acid) is the genetic material of almost all living organisms. It is a double…

Q: What are three major limitations of forensic DNA profiling?

A: DNA profiling is the process of obtaining a unique profile from the DNA of an individual. This…

Q: What are the steps of a PCR?

A: PCR stands for Polymerase Chain Reaction. It is a technique of molecular biology which is used to…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.