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- You are studying the binding of proteins to thecytoplasmic face of cultured neuroblastoma cells andhave found a method that gives a good yield of inside-outvesicles from the plasma membrane. Unfortunately, yourpreparations are contaminated with variable amounts ofright-side-out vesicles. Nothing you have tried avoids thisproblem. A friend suggests that you pass your vesicles overan affinity column made of lectin coupled to solid beads.What is the point of your friend’s suggestion?Both H+ and Ca2+ are ions that move through thecytosol. Why is the movement of H+ ions so much fasterthan that of Ca2+ ions? How do you suppose the speed ofthese two ions would be affected by freezing the solution?Would you expect them to move faster or slower? Explainyour answer.Imagine protein X, destined to span theplasma membrane. Assume that the mRNA carrying the geneticmessage for protein X has already been translated by ribosomesin a cell culture. If you fractionate the cells (see Figure 6.4), inwhich fraction would you find protein X? Explain by describingits transit through the cell.
- Hello, In the provided picture I am to select the FIVE compunds that would be the most stable in a CELL MEMBRANE. I had selected A,B,C,D,and E and recieved 1.2 out of 2. Can you please help!!! Thank you so MuchSuppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl(~0.03 M). When isolating lysosomes, what concentration of sucrose isrequired in the extracting solution at room temperature (25 °C) to preventswelling and lysis?Rb is a negative regulator that blocks the cell cycle at the G1 checkpoint until the cell achieves a requisite size. What molecular mechanism does Rb employ to halt the cell cycle?