Please match the following definitions to the correct termsB-galactosidase[ Choose ]lactose permease[ Choose ]Lac I[ Choose ]Lac Y[ Choose ]operator[Choose ]Lạc Z[ Choose ]operon[ Choose ]trans-acting[ Choose ]cis-acting[ Choose ]negative regulation[ Choose ]>>>>>> Proteins that are soluble and can move from one DNA strand to another are calledWhen a repressor protein binds to the operator to prevent transcriptionThe gene that encodes B-galactosidaseThe gene that encodes lactose permeaseA segment of DNA found between the promoter and structural genes that acts as a control elementThe transmembrane protein that allows lactose to pass through the cell membrane an into the cellThe gene that encodes for a lac operon repressor proteinRegions of DNA that do not move and control expression locally are calledpositive regulationA set of linked genes under the control of the same promoterThe enzyme that breaks down lactose into galactose and glucose

Name: Please match the following definitions to the correct termsB-galactos
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Description: Please match the following definitions to the correct termsB-galactosidase[ Choose ]lactose permease[ Choose ]Lac I[ Choose ]Lac Y[ Choose ]operator[Choose ]Lạc Z[ Choose ]operon[ Choose ]trans-acting[ Choose ]cis-acting[ Choose ]negative regulation

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Biology

Please match the following definitions to the correct terms B-galactosidase [ Choose ] lactose permease [ Choose ] Lạc I [ Choose ] > >

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 21P

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It has been postulated that thiogalactoside transacetylase (LacA in the lactose operon) plays a role in detoxification by acetylating potentially hazardous -galactosides to prevent their absorption after they have diffused out of the cell. How would you put this idea to the test?
. Seven E. coli mutants were isolated. The activity ofthe enzyme β-galactosidase produced by cells containing each mutation alone or in combination with othermutations was measured when the cells were grown inmedium with different carbon sources.Lactose +Glycerol Lactose GlucoseWild type 0 1000 10Mutant 1 0 10 10Mutant 2 0 10 10Mutant 3 0 0 0Mutant 4 0 0 0Mutant 5 1000 1000 10Mutant 6 1000 1000 10Mutant 7 0 1000 10F′ lac from mutant 0 1000 101/ mutant 3F′ lac from mutant 0 10 102/ mutant 3Mutants 3 + 7 0 1000 10Mutants 4 + 7 0 0 0Mutants 5 + 7 0 1000 10Mutants 6 + 7 1000 1000 10Assume that each of the seven mutations is one andonly one of the genetic lesions in the following list.Identify the type of alteration each mutation represents.a. superrepressorb. operator deletionc. nonsense (amber) suppressor tRNA gene (assumethat the suppressor tRNA is 100% efficient in suppressing amber mutations)d. defective CRP–cAMP binding sitee. nonsense (amber) mutation in the β-galactosidase genef.…
By using separate labelled diagrams, describe as detail as possile the actions of the normal and mutated lac operon in absence of lactose.
The symbols a, b, and c in the table below represent theE. coli lac system genes for the repressor (I ), the operator(O) region, and the β-galactosidase (Z), although notnecessarily in that order. Furthermore, the order inwhich the symbols are written in the genotypes is notnecessarily the actual sequence in the lac operon.Activity (+) or inactivity (−) of Z geneGenotype Inducer absent Inducer presenta- b+ c+ + +a+ b+ c- + +a+ b- c- - a+ b- c+/a- b+ c- + +a+ b+ c+/a- b- c- - +a+ b+ c-/a- b- c+ - +a- b+ c+/a+ b- c- + +a. Which symbol (a, b, or c) represents each of the lacgenes I, O, and Z ?b. In the table, a superscript minus sign on a gene symbolmerely indicates a mutant, but some mutant behaviorsin this system are given special mutant designations.Using the conventional gene symbols for the lac operon,designate each genotype in the table.
Which of the following lac operon genotypes would allow for functional versions of all the structural enzymes of the lac operon to be expressed constitutively even in the absence of lactose? Group of answer choices I+ O+ Z+ Y+ A+ I- O+ Z- Y- A- I+ OC Z+ Y+ A+ IS O+ Z+ Y+ A+ I+ O+ Z- Y+ A+
Given the following genotypes, explain, by answering the questions in each number, how the mutation (identified by a (-) superscript) will affect E. coli grown in lactose medium. Will there be a complete set ofgene products? (Yes/No) Will the lac operon be turnedon/off? Will the cell survive? (Yes/No) a. i + p + o + z - y + b. i + p - o + z + y + c. i + p + o - z + y +
Based on your knowledge of allosteric regulation, invent and describe a hypothetical mutation that would produce constitutive expression of the Lac operon but is not a mutation that occurs in the Lac operator and is not a mutation to the DNA binding domain of LacI. Clearly mention where in the prokaryotic genome this mutation would occur and explain the logic behind how that mutation would produce constitutive Lac operon expression.
Suppose that you have isolated a mutant strain of E. coli inwhich the lac operon is constitutively expressed. To understandthe nature of this defect, you create a merozygote in which themutant strain contains an F′ factor with a normal lac operonand a normal lacI gene. You then compare the mutant strain andthe merozygote with regard to their β-galactosidase activities inthe presence and absence of lactose. You obtain the followingresults:
Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.
Please complete the table representing the regulation of the lactose operon. Please highlight any data that provides evidence of trans regulation.
Mutations in the genes of the lac operon might affect the regulation of β-galactosidase synthesis. For each mutation listed below, indicating whether β-galactosidase would be regulated normally, always ON or always OFF. Mutation in operator site prevents repressor binding Mutation in lacIgene prevents repressor from binding operator Mutation in lacIgene prevents repressor from binding allolactose Nonsense mutation in lacZgenere
Which of the following correctly describes the regulation and activity of HIF-1? Group of answer choices VHL is required for preventing degradation of HIF-1alpha and promoting translocation to the nucleus In normoxia, HIF-1alpha is ubquitinated and degraded in the proteasome Hypoxic conditions result in increase hydroxylation of prolines in HIF-1alpha The primary function of HIF-1 is to phosphorylate and activate glycolytic enzymes