. Seven E. coli mutants were isolated. The activity ofthe enzyme β-galactosidase produced by cells containing each mutation alone or in combination with othermutations was measured when the cells were grown inmedium with different carbon sources.Lactose +Glycerol Lactose GlucoseWild type 0 1000 10Mutant 1 0 10 10Mutant 2 0 10 10Mutant 3 0 0 0Mutant 4 0 0 0Mutant 5 1000 1000 10Mutant 6 1000 1000 10Mutant 7 0 1000 10F′ lac from mutant 0 1000 101/ mutant 3F′ lac from mutant 0 10 102/ mutant 3Mutants 3 + 7 0 1000 10Mutants 4 + 7 0 0 0Mutants 5 + 7 0 1000 10Mutants 6 + 7 1000 1000 10Assume that each of the seven mutations is one andonly one of the genetic lesions in the following list.Identify the type of alteration each mutation represents.a. superrepressorb. operator deletionc. nonsense (amber) suppressor tRNA gene (assumethat the suppressor tRNA is 100% efficient in suppressing amber mutations)d. defective CRP–cAMP binding sitee. nonsense (amber) mutation in the β-galactosidase genef. nonsense (amber) mutation in the repressor geneg. defective crp gene (encoding the CRP protein)
. Seven E. coli mutants were isolated. The activity of
the enzyme β-galactosidase produced by cells containing each mutation alone or in combination with other
mutations was measured when the cells were grown in
medium with different carbon sources.
Lactose +
Glycerol Lactose Glucose
Wild type 0 1000 10
Mutant 1 0 10 10
Mutant 2 0 10 10
Mutant 3 0 0 0
Mutant 4 0 0 0
Mutant 5 1000 1000 10
Mutant 6 1000 1000 10
Mutant 7 0 1000 10
F′ lac from mutant 0 1000 10
1/ mutant 3
F′ lac from mutant 0 10 10
2/ mutant 3
Mutants 3 + 7 0 1000 10
Mutants 4 + 7 0 0 0
Mutants 5 + 7 0 1000 10
Mutants 6 + 7 1000 1000 10
Assume that each of the seven mutations is one and
only one of the genetic lesions in the following list.
Identify the type of alteration each mutation represents.
a. superrepressor
b. operator deletion
c. nonsense (amber) suppressor tRNA gene (assume
that the suppressor tRNA is 100% efficient in suppressing amber mutations)
d. defective CRP–cAMP binding site
e. nonsense (amber) mutation in the β-galactosidase gene
f. nonsense (amber) mutation in the repressor gene
g. defective crp gene (encoding the CRP protein)
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