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- 4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.A mixture of seven amino acids (glycine, glutamate, leucine, lysine, alanine, isoleucine, and aspartate) is separated by chromatography. Explain why only six spots show up when the chromatographic plate is coated with ninhydrin and heated.In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 4 cm away from the finish line. The distance travelled from the starting line to finish line is 40 cm. The first amino acid, AA1 travelled 6 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 8 cm ahead of AA2. Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____ (Rf= Retention factor)
- A solution containing orange rind extract was analyzed using gas chromatography to measure its limonene content. The parameters used to analyze the standards and samples are listed below. Calculate the concentration of limonene in the solution in ug/mLUpon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of your answers on the spots in the chromatogram shown at the right.What is chiral chromatography?
- Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate the mixture using a column chromatography technique. He packed the column with amino bonded with siloxane material and use a mixture of 20:80 of water: hexane as the mobile phase. i) Sketch the apparatus set up for the chromatographic technique used by Iman. Use the correct chromatographic terminologies to label all of the apparatus, materials, and phases. ii) Predict the sequence of elution when the mixture was loaded onto the separation column. Justify your answer. iii) After the first compound was eluted, Iman discovered that the second compound was taking too long to pass through the column. Please suggest any strategies that could be used to aid the elution process.You were analyzing a mixture of volatile solvents using Gas Chromatography and found out that two peaks are unresolved (not well-separated) using your initial program. Which method can you use to obtain a chromatogram with well separated peaks?Determination of ascorbic acid in vitamin C tablets: which analytical approach is better; volumetric or spectrophotometric analysis?
- Amino acids and fatty acids do not readily elute from gas chromatography columns even at temperatures above 200 degrees Celsius. What can be done to these biomolecules to allow gas chromatographic analysis?Briefly explain how cation exchange chromatography can used in protein separation?Separation of Amino Acids by Thin Layer Chromatography Lab Questions. Please see photos and answer the questions relating to the images from #10. Thank you!