10. Consider the following compounds when answering the questions below. Assume these compounds can be separated by TLC. A B OH C Separation of Amino Acids by Thin Layer Chromatography a. Arrange the above compounds in order of increasing Re in a TLC analysis based on their structure and how they adsorption and separation on TLC plate. (Hint: Background information document: What separates the compounds as a chromatogram develops?)

EBK A SMALL SCALE APPROACH TO ORGANIC L
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ISBN:9781305446021
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Chapter89: Thin-layer Chromatography
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Separation of Amino Acids by Thin Layer Chromatography Lab Questions. Please see photos and answer the questions relating to the images from #10. Thank you!

11. A TLC plate is spotted with each of the three compounds. The plate is developed using hexanes:
ethyl acetate (5:95) to give the chromatogram shown below. How could you change the solvent system
to give better separation of these three compounds?
12. Arrange the following in order of increasing R, on thin-layer chromatography analysis plate based on
their structure and how they adsorp and separate on TLC plate: Octanoic acid, Butyraldehyde, 2-
Octanone, Decane, and 1-pentanol. (Hint: Background information document: What separates the
compounds as a chromatogram develops?)
13. You are trying to determine TLC solvent system which will separate the compounds X, Yand Z. you
ran a TLC plate using hexane/ ethyl acetate 95:5 as the eluting solvent and obtained the chromatogram
below. How would you change the solvent system to give better separtion of these three compounds?
-0-0-0
Transcribed Image Text:11. A TLC plate is spotted with each of the three compounds. The plate is developed using hexanes: ethyl acetate (5:95) to give the chromatogram shown below. How could you change the solvent system to give better separation of these three compounds? 12. Arrange the following in order of increasing R, on thin-layer chromatography analysis plate based on their structure and how they adsorp and separate on TLC plate: Octanoic acid, Butyraldehyde, 2- Octanone, Decane, and 1-pentanol. (Hint: Background information document: What separates the compounds as a chromatogram develops?) 13. You are trying to determine TLC solvent system which will separate the compounds X, Yand Z. you ran a TLC plate using hexane/ ethyl acetate 95:5 as the eluting solvent and obtained the chromatogram below. How would you change the solvent system to give better separtion of these three compounds? -0-0-0
10. Consider the following compounds when answering the questions below. Assume these
compounds can be separated by TLC.
A
B
OH
C
Separation of Amino Acids by Thin Layer Chromatography
a. Arrange the above compounds in order of increasing Rf in a TLC analysis based on their structure and
how they adsorption and separation on TLC plate. (Hint: Background information document: What
separates the compounds as a chromatogram develops?)
Transcribed Image Text:10. Consider the following compounds when answering the questions below. Assume these compounds can be separated by TLC. A B OH C Separation of Amino Acids by Thin Layer Chromatography a. Arrange the above compounds in order of increasing Rf in a TLC analysis based on their structure and how they adsorption and separation on TLC plate. (Hint: Background information document: What separates the compounds as a chromatogram develops?)
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