Poble un Set 4 10. (10 points) Your group is interested in synthesizing a new class of bacterial antibiotics that specifically targets that translational machinery of a prokaryotic organism, the 70S ribosome. As a first step in this study your group has just assigned you to the purification of these ribosomes. After this exhaustive work you have come to the point in the purification where you must quantify your samples. A 1:1000 70S to buffer ratio for spectrophotometric analysis resulted in 0.7690 A26o units. If 1 A26o unit is equal to 23 pmol of 70S ribosomes, and you have a sample volume size of 500 uL what is the concentration of your sample in HM. 11. (30 points) While you were purifying the 70S ribosomes, your lab mates were synthesizing potential inhibitors for screening. They have made many inhibitors but one compound in particular shows promise in in-vivo work but must a 1 mg sample of compound X-A2P67 (MW 54448) Your lab mate informs you that in order to achieve optimal stability and solubility yo 1 %,DMSO. Whatstheconcentration of compound Xin molarity(M m or n order to estimate a minimum inhibitory concentratton X, it is best to perform a drug titration assay conce in vitro as well. Your lab mate just gave you p should be to dissolve compound X in 1 mL of by compound e nd X nM, 500 nM, 1 μΜ, 2μΜ and 5 μΜ. The total reaction anslation o ecide following compou ssay: 100 n volume for each translation assay 16 25 L. Your inhibitor content for each reaction must b Also note that all other dilutions after making your initial stock of compound X in DMSO M in water to avoid total protein unfolding. Your total DMSO content in each reaction must not exceed 2.596. Make a table of how you would designth experiment Showatotyour work

Question

I am not sure how to do number 10

Poble un Set 4
10. (10 points) Your group is interested in synthesizing a new class of bacterial antibiotics that
specifically targets that translational machinery of a prokaryotic organism, the 70S ribosome. As a
first step in this study your group has just assigned you to the purification of these ribosomes. After
this exhaustive work you have come to the point in the purification where you must quantify your
samples. A 1:1000 70S to buffer ratio for spectrophotometric analysis resulted in 0.7690 A26o units.
If 1 A26o unit is equal to 23 pmol of 70S ribosomes, and you have a sample volume size of 500 uL
what is the concentration of your sample in HM.
11. (30 points) While you were purifying the 70S ribosomes, your lab mates were synthesizing
potential inhibitors for screening. They have made many inhibitors but one compound in particular
shows promise in in-vivo work but must
a 1 mg sample of compound X-A2P67 (MW 54448) Your lab mate informs you that in order to
achieve optimal stability and solubility yo
1 %,DMSO. Whatstheconcentration of compound Xin molarity(M m or n order to
estimate a minimum inhibitory concentratton
X, it is best to perform a drug titration assay
conce
in vitro as well. Your lab mate just gave you
p should be to dissolve compound X in 1 mL of
by compound
e nd X
nM, 500 nM, 1 μΜ, 2μΜ and 5 μΜ. The total reaction
anslation o
ecide
following compou
ssay: 100 n
volume for each translation assay 16 25 L. Your inhibitor content for each reaction must b
Also note that all other dilutions after making your initial stock of compound X in DMSO M
in water to avoid total protein unfolding. Your total DMSO content in each reaction must not exceed
2.596. Make a table of how you would designth experiment Showatotyour work
Expand
Transcribed Image Text

Poble un Set 4 10. (10 points) Your group is interested in synthesizing a new class of bacterial antibiotics that specifically targets that translational machinery of a prokaryotic organism, the 70S ribosome. As a first step in this study your group has just assigned you to the purification of these ribosomes. After this exhaustive work you have come to the point in the purification where you must quantify your samples. A 1:1000 70S to buffer ratio for spectrophotometric analysis resulted in 0.7690 A26o units. If 1 A26o unit is equal to 23 pmol of 70S ribosomes, and you have a sample volume size of 500 uL what is the concentration of your sample in HM. 11. (30 points) While you were purifying the 70S ribosomes, your lab mates were synthesizing potential inhibitors for screening. They have made many inhibitors but one compound in particular shows promise in in-vivo work but must a 1 mg sample of compound X-A2P67 (MW 54448) Your lab mate informs you that in order to achieve optimal stability and solubility yo 1 %,DMSO. Whatstheconcentration of compound Xin molarity(M m or n order to estimate a minimum inhibitory concentratton X, it is best to perform a drug titration assay conce in vitro as well. Your lab mate just gave you p should be to dissolve compound X in 1 mL of by compound e nd X nM, 500 nM, 1 μΜ, 2μΜ and 5 μΜ. The total reaction anslation o ecide following compou ssay: 100 n volume for each translation assay 16 25 L. Your inhibitor content for each reaction must b Also note that all other dilutions after making your initial stock of compound X in DMSO M in water to avoid total protein unfolding. Your total DMSO content in each reaction must not exceed 2.596. Make a table of how you would designth experiment Showatotyour work

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