Problem 3. (6.27, From Chang's Book). The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment: Total Ca2+ (uM) 60 120 180 240 480 Ca2+ bound to Protein (uM) 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96 µM for each run. (1 uM = 1 x 10-6 M.)
Problem 3. (6.27, From Chang's Book). The calcium ion binds to a certain protein to form a 1:1 complex. The following data were obtained in an experiment: Total Ca2+ (uM) 60 120 180 240 480 Ca2+ bound to Protein (uM) 31.2 51.2 63.4 70.8 83.4 Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96 µM for each run. (1 uM = 1 x 10-6 M.)
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
Related questions
Question
Transcribed Image Text:Problem 3. (6.27, From Chang's Book). The calcium ion binds to a certain protein to form a 1:1 complex. The following data
were obtained in an experiment:
Total Ca2+ (uM)
60
120
180
240
480
Ca2+ bound to Protein (uM)
31.2 51.2 63.4
70.8
83.4
Determine graphically the dissociation constant of the Ca2+ - protein complex. The protein concentration was kept at 96 µM
for each run. (1 uM = 1 x 10-6 M.)
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemical-engineering and related others by exploring similar questions and additional content below.Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The