Procedure Mix Take ml Top up to 50ml. Add 50 ul of Sml mixture Add fresh LB ampicillin to the overnight culture with 45 ml LB to mixture analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until Overnight culture incubated analyse (OD600)record as shaked incubator at 37°c 200rpm at (E-coli + LB borth) zero time-point. 75 mins. Mix overnight culture Sml Take 3 ml No ampicillin - mixture analyse to No ampicilin B (control) with 45 ml LB broth the OD600 Withdraw 3ml mixture to The incubated incubator at 37°c 200rpm mixture Measure every 15 mins. Stop until is then shaked -analyse (OD600)record as at zero time-point. 75 mins. Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 2 (Ampicillin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)

Procedure Mix Take ml Top up to 50ml. Add 50 ul of Sml mixture Add fresh LB ampicillin to the overnight culture with 45 ml LB to mixture analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until Overnight culture incubated analyse (OD600)record as shaked incubator at 37°c 200rpm at (E-coli + LB borth) zero time-point. 75 mins. Mix overnight culture Sml Take 3 ml No ampicillin - mixture analyse to No ampicilin B (control) with 45 ml LB broth the OD600 Withdraw 3ml mixture to The incubated incubator at 37°c 200rpm mixture Measure every 15 mins. Stop until is then shaked -analyse (OD600)record as at zero time-point. 75 mins. Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 2 (Ampicillin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.7: The Metric System

Problem 1P

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