nna is training to be a cell culture technician. She uses some sterile distilled water to wasg a batch of cell culture plates. When she looks at the cell culture plates under the microscope to check the cells after this, she notices the cell have burst. She realizes she should have used 0.9% saline instead. Explain what has happened, and why she should have used the saline.
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Anna is training to be a cell culture technician. She uses some sterile distilled water to wasg a batch of cell culture plates. When she looks at the cell culture plates under the microscope to check the cells after this, she notices the cell have burst. She realizes she should have used 0.9% saline instead. Explain what has happened, and why she should have used the saline.
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- How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell at the same time? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/What is the biological benefit(s) to the organism to have detachment induced cell death? Please explain as much as possible (at least 5 sentences)Why must primary cell cultures be restarted every so often when preparing primary cell cultures to observe morphological changes caused by cells infected by a virus? Why are tumor cells preferred?
- In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are counted. Answer the question below. there were _____cells in the 1 mL of culture that was spread on the plate. If the culture was diluted 1:100 prior to plating, how many cells were there per mL in the original culture? _________When Miescher added the alkaline solution, the cells lysed. What does this mean? A. They mutliplied. B. They became stronger. C. They broke open. D. They froze in time.Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in tube-A, then transferred 10 µL to the hemocytometer and started counting cells, then realized the cells were too crowded to count accurately, so Steven took an aliquot of 20 µL cells of cells from tube-A and mixed with 180 µL of cell culture media, transferred 10 µL onto the hemocytometer, and countered 500 cells from the squares on the four corners of the hemacytometer. What is the # of cells per mL? Please show all calculation steps.
- How would I figure out how many dilutions to make given cell density ? And volume to plate ? For example, I want to plate 0.1 ml and want 200 colonies how many dilutions would i need to make, given 7.5 x10^5 cells/ mlGrowing cells outside the body needs laboratory space, aseptic techniques and special equipments such as incubator. The terminology use to say growing cells in lab is calledFeatures of continuous cell culture include the following, except ________ . a.addition of product b. stirring c.addition of nutrients d.removal of product e.sterilization of nutrients
- Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. At 100X you are unable to see the diatom. Explain why. Explain what correction (s) should be made to allow you to see the diatom using the 100X lens. After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred.Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution using only 4.5 mL diluents in tubes. What would be the theoretical cell count if 0.1 mL of the 2nd and 4th dilutions were each plated?Your first task in the lab is to learn how to culture these cells and begin expanding the culture (increasing the # of cells) so that you may later conduct experiments on them. You take two aliquots, each with an equal number of cells, and place them in two different culture vessels, one is a sealed airtight flask (culture 1) and the other is an open topped culture dish (culture 2). You provide both with the same growth media that contains their preferred fuel source, glucose. Will one of these cultures (#1 or #2 as described above) need to have their media changed more frequently than the other? (Yes or No) If yes, which culture will need to have its media changed more often and why? If no, why not?