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Curren'S Math For Meds: Dosages & Sol

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 42SST

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Protein Solubility
3.5
5
0.028
Absorbance 0.098
Conc.(mg/ml) 0.195
0.044
0.9%
2.786%
9.429%
11.3:
% solubility 4.179%
The above table indicates the concentration of protein in the diluted supernatant and the
supernatant before dilution at different pH.
Dilution Factor
30
22.5
pH
15
7.5
1.5
4.5
5
0.027
3
0.042
5.5
5
0.068
0.130
Protein Solubility Versus pH
6
6.5
4.5
pH Value
The above figure shows a plot of protein solubility versus pH.
50
Please provide a brief discussion and explanation of the results.
7.5
7.5
50
0.032
0.053
9
8.5
50
0.054
0.100
21.429%

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pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))
pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the amount of soluble protein (in grams) in the soy flour extract
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