Q.1 The spectrum shown could represent the molecule in the illustration H3C -OH 8 H3C to کر کر کہ 2
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![Q.1 The spectrum shown could represent the molecule in the illustration
H3C
-OH
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H3C
to
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کر
کہ
2](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F72fedc53-419f-47ee-aa8e-bfe5f76f9d77%2F47adecad-a21f-4ada-8823-bd3c2f1182b6%2F8e5ugv_processed.png&w=3840&q=75)
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- Nonspecific elution of affinity bonded macromolecules is used in affinity chromatography explain why?12 Determine the type of biochemical reaction that occurs in the reaction shown below. O O O O O redox isomerization group C-C bond formation/cleavage transfer hydrolysis/condensation Previous Ex-xx-xx OH (ATP) OH OH OH OH + AMP OH OPO₂² OH OH +AMP1 OH (ADP)CHEM151/251 Biochemistry 1 a. Determine Km 1. The following data were obtained for a competitive inhibition study in which the [I] = 3 µM for each determination of vo in the presence of inhibitor. The Vmax = 200 μM P/min for both data sets. 200 Vo (UM P/min) 8 8 8 8 8 8 8 8 8 180 160 140 120 100 40 Name (Print)/ID #: the absence of inhibitor. Participation Question # 10 No Inhibitor 50 +Inhibitor 100 [Substrate] (UM) 150 b. Determine Km, app for the data obtained in presence of inhibitor. 200 c. Calculate the value for Ki. Note: a = 1 + [I]/Ki and a¹ = 1 + [I]/Ki*. for the data obtained in
- 2c which is altered by a catalyst such as an enzyme, the net free energy change of the reaction or the activation energy of the reaction?8:05 Expert Q&A Done A bioinformatics study revealed that the enzymes called vasoinhibins have a Cys-His-Ser catalytic triad. These enzymes regulate angiogenesis (the formation of new blood vessels) and are thus potential drug targets for cancer treatment. If the cysteine residue serves as the nucleophile, what are the possible roles of histidine and serine in enzyme catalysis?how are SDS-PAGE and BN-PAGE techniques used determine the composition of protein-photosynthetic complexes? also how do they work? please be thorough in the explanation!
- 1:55 .ll 0.52 KB/S 56 1.pdf CHEM 3141: BioChemistry Quiz no. 1- Answer key I.) Draw the following compounds. 1.) Maltose: 2 Glucose, a-(1,4) 2.) Gentobiose: 2 Glucose, B-(1,6) 3.) C-2 epimer of Talose 4.) C-3 epimer of Fructose 5.) C-2 epimer of ribose II. Predict the products of the following reactions, if there is none, write NO RXN. Also indicate, if the reaction is fast or slow он FO HO -H H -OH но. + Resorcinol/dilute HCI 1.) 2.) Sucrose + Copper (II) Acetate/Acetic Acid HO H HO H HO H H OH 3.) HO + Orcinol/HCI3 Acra Alrich TOI NOF 200 250 300 350 Wavelength (nm) UV/Vis spectrum of four brands of polysorbate 80, a common detergent used in protein purification. From Wuelfin, et al., "Polysorbate 80 UV/vis spectral and chromatographic characteristics-defining boundary conditions for use of the surfactant in dissolution analysis." 3. While the binding of Coomassie blue to proteins can lead to accurate measurements of protein concentration, there are situations where the absorbance values could be misleading. For example, detergents are often used in the purification of proteins. Certain detergents mixed in with the dye could lead to incorrect determinations of protein concentration. Take a look at the spectrum of the detergent polysorbate 80, above. If you had used this detergent to purify the protein in part c above, would you expect it to interfere with your concentration calculations? Why or why not? Absorbance (AU)) To better understand how the lacR works, let's start let's approximate the volume of a bacterial cell by treating it as a cylinder with radius that is 0.5 um and length that is 2 um. What is the volume of the cylinder in liters, L)? Next, let's calculate the concentrations of the lacR (ligand) and the operator site (Op) (primary receptor) in this volume. There are 10 lacR molecules in the cell and 1 operator site. Using this information calculate [lacR] and [Op] in molar units in one cell? (see eq. 13.17 and 13.18).
- 7. 1 B e here to search Required Study the two diagrams below. Diagram A 2 1 Diagram B 2 Based on the sequence of steps (1, 2, 3), which of the diagrams shows what would happen to proteins made at the ribosomes and transported to the outside of the cell? Diagram A Diagram B Both Diagram A and Diagram B Neither Diagram A nor Diagram B DELL 40)Why is ΔG a useful quantity for predicting the favorability of biochemical reactions?Özcan MM. and his colleagues tried to obtain 3 different fatty acids from moringa pelegrina nucleus with cold pressing and soxhlet extraction by two different methods. The data is as follows; Fatty acids Cold pressing method Moringa pelegrina nucleus Soxhlet extraction method Moringa pelegrina nucleus a-tocopherol(mg/kg) 137.89 ± 2.89 y-tocopherol(mg/kg) 63.68+0.87 8-tocopherol(mg/kg) 151.67+1.89 71.57+1.06 61.49+0.76 63.58+0,97 Accordingly, 3 different fatty acids obtained by 2 different methods used; a) Calculate their efficiency. b) By which method has more efficiency been achieved and explain with the reasons?
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