Qualitative and Quantitative Tests for Amino Acids and Proteins Complete the table: Test Reagents Positive Result Positive Indication Ninhydrin test Xanthoproteic acid test Millon's test Hopkins Cole test Pauly's diazo Test Lead sulphide test Histidine test
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Qualitative and Quantitative Tests for Amino Acids and Proteins
Complete the table:
|
Test |
Reagents |
Positive Result |
Positive Indication |
|
Ninhydrin test |
|
|
|
|
Xanthoproteic acid test |
|
|
|
|
Millon's test |
|
|
|
|
Hopkins Cole test |
|
|
|
|
Pauly's diazo Test |
|
|
|
|
Lead sulphide test |
|
|
|
|
Histidine test |
|
|
|
|
Isatin test |
|
|
|
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Folin’s McCarthy Sullivan Test |
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Nitroprusside Test |
|
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- Create an illustrated step-by-step schematic diagram of the following Qualitative test for Lipids: • Solubility Test• Translucent Test• Acrolein Test• Baudouin Test• Huble’s TestCreate an illustrated step-by-step schematic diagram of the following Qualitative test for Lipids: Baudouin Test Huble’s TestBased on the following qualitative tests for proteins, which one is positive/negative for intact protein, acid hydrolysate, and base hydrolysate? Biuret Test Ninhydrin test Sakaguchi test Xanthoprotein test Hopkins-Cole test Lead acetate test Millon’s test
- Which of the different carbohydrate tests would give a positive result for maltose? Describe the color change. Molisch test Fehling’s test Benedict’s test Tollen’s Test Barfoed’s test Seliwanoff’s test Phenylhydrazine or osazone testGive and explain the principles of the following tests for lipids: Spot Test Acrolein Test Test for Free glycerol Test for Surface Tension Formation of Insoluble Soap Liebermann-Burchard Test Salkowski TestProvide the Principles and detailed procedures of the following tests for the "Color Reaction of Proteins". 1. Hopkins-Cole Test 2. Sulfur Test 3. Heller's Test
- The table below summarizes the results for Millon's test. Provide the correct remarks from the results if Negative for Millon's Test or Positive for Millon's Test and if Contains phenolic hydroxy group or Does not contains phenolic hydroxy group.Give at least two (2) chemical tests for amino acids/ proteins other than what were performed in this experiment. For each test, cite the respective application and describe the positive result. a.Ninhydrin Test b.Biuret’s Test c.Xanthoproteic Test d.Millon’s Test e.Sakaguchi test f.Hopkins –Cole Test g.Sulfur Reaction or Lead Acetate TestThe table below summarizes the results for Hopkins-Cole test. Provide the correct remarks from the results if Negative for Hopkin's Cole test or Positive for Hopkin's Cole test and if Negative for indole group or Has the presence of indole group.
- In a tabular form, enumerate other chemical qualitative tests for carbohydrates, discuss its principle, chemical reagents employed and its positive test result.Give at least two (2) chemical tests for amino acids/ proteins other than what were performed in this experiment. For each test, cite the respective application and describe the positive result. test that have been performed a.Ninhydrin Test b.Biuret’s Test c.Xanthoproteic Test d.Millon’s Test e.Sakaguchi test f.Hopkins –Cole Test g.Sulfur Reaction or Lead Acetate TestDraw the chemical reaction involved in each qualitative reactions of proteins. Ninhydrin test (ninhydrin solution) Xanthoproteic test (concentrated nitric acid) Millon’s test (Millon's reagent) Hopkins-Cole test (glyoxylic acid) Biuret test (Biuret solution)