Question #1: In 1966, a mouse was found in the Jackson Laboratory colony that seemed to be excessive in weight as compared to its wildtype siblings from the same litter. The allele that was assigned to this mouse was called "db". Given the data from the original 1966 paper below, a) is there evidence of a genetic basis for an increased overall weight of the mutant mouse? b) Is this mutant phenotype encoded by a dominant or recessive gene? Please provide an explanation of your answers. Table 1. Body weight of diabetic (dbdb) and normal (++ and + db) mice. Sexes are combined. Age (weeks) 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 11-12 12-13 13-22 23-54 No. of mice 6 12 25 20 16 12 10 11 11 7 5 11 5 dbdb Av. 6.7 14.8 19.8 25.4 28.3 32.4 34.5 37.9 39.4 38.1 44.4 45.3 45.9 Weight (g) Range AG GŤAAAC 7-10 9- 20 10- 27 14- 34 last two nucleotides of the 106 bp 18- 39 21- 42 31-41 31-44 32- 45 32-46 34-51 32- 62 37-61 No. of mice 7 21 16 19 17 17 5 26 ++and+ db Av. 7.6 11.3 16.9 18.5 19.8 21.9 27.3 28.9 ...exon...AG GTAAGT...intron.. Weight (g) In 1996, the genetic basis this mutation was mapped to leptin gene and no large chromosomal variations were found in the db variant or around the leptin gene as compared to wildtype siblings. c) What was the likely mechanism by which this mutation arose in the mutant mouse? Range 7- 9 9-13 15- 21 15- 23 16- 28 19- 28 Also in 1996, the genetic basis of the change was found to be a mutation in the splice site of the exon, with a single mutation of G to T. d) What was the likely lab technique used to identify this nucleotide change? AG GGAAAC 20-36 20-38 wt/wt db/db consensus
Question #1: In 1966, a mouse was found in the Jackson Laboratory colony that seemed to be excessive in weight as compared to its wildtype siblings from the same litter. The allele that was assigned to this mouse was called "db". Given the data from the original 1966 paper below, a) is there evidence of a genetic basis for an increased overall weight of the mutant mouse? b) Is this mutant phenotype encoded by a dominant or recessive gene? Please provide an explanation of your answers. Table 1. Body weight of diabetic (dbdb) and normal (++ and + db) mice. Sexes are combined. Age (weeks) 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 11-12 12-13 13-22 23-54 No. of mice 6 12 25 20 16 12 10 11 11 7 5 11 5 dbdb Av. 6.7 14.8 19.8 25.4 28.3 32.4 34.5 37.9 39.4 38.1 44.4 45.3 45.9 Weight (g) Range AG GŤAAAC 7-10 9- 20 10- 27 14- 34 last two nucleotides of the 106 bp 18- 39 21- 42 31-41 31-44 32- 45 32-46 34-51 32- 62 37-61 No. of mice 7 21 16 19 17 17 5 26 ++and+ db Av. 7.6 11.3 16.9 18.5 19.8 21.9 27.3 28.9 ...exon...AG GTAAGT...intron.. Weight (g) In 1996, the genetic basis this mutation was mapped to leptin gene and no large chromosomal variations were found in the db variant or around the leptin gene as compared to wildtype siblings. c) What was the likely mechanism by which this mutation arose in the mutant mouse? Range 7- 9 9-13 15- 21 15- 23 16- 28 19- 28 Also in 1996, the genetic basis of the change was found to be a mutation in the splice site of the exon, with a single mutation of G to T. d) What was the likely lab technique used to identify this nucleotide change? AG GGAAAC 20-36 20-38 wt/wt db/db consensus
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter13: Artifacts And Noise In Medical Instrumentation
Section: Chapter Questions
Problem 7P
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Need help with parts a,b and c, please and thank you!
Transcribed Image Text:Question #1: In 1966, a mouse was found in the Jackson Laboratory colony that seemed to be
excessive in weight as compared to its wildtype siblings from the same litter. The allele that was
assigned to this mouse was called "db".
Given the data from the original 1966 paper below, a) is there evidence of a genetic basis for
an increased overall weight of the mutant mouse? b) Is this mutant phenotype encoded
by a dominant or recessive gene? Please provide an explanation of your answers.
Table 1. Body weight of diabetic (dbdb) and normal (++ and + db) mice. Sexes are
combined.
Age
(weeks)
2-3
3- 4
4-5
5-6
6-7
7-8
8-9
9-10
10-11
11-12
12-13
13-22
23-54
No.
of
mice.
6
12
25
20
16
12
10
11
11
7
5
11
5
dbdb
Av.
6.7
14.8
19.8
25.4
28.3
32.4
34.5
37.9
39,4
38.1
44.4
45.3
45.9
Weight (g)
AG GTAAAC
Range
7- 10
9- 20
last two nucleotides of the 106 bp
10- 27
14- 34
18-39
21-42
31-41
31-44
BONBO
32- 45
32-46
34-51
32- 62
37-61
No.
of
mice
7
21
16
19
17
17
5
26
++and+ db
Av.
7.6
11.3
16.9
18.5
19.8
21.9
27.3
28.9
In 1996, the genetic basis of this mutation was mapped to the leptin gene and no large
chromosomal variations were found in the db variant or around the leptin gene as compared to
wildtype siblings. c) What was the likely mechanism by which this mutation arose in the
mutant mouse?
Weight (g)
Also in 1996, the genetic basis of the change was found to be a mutation in the splice site of the
exon, with a single mutation of G to T. d) What was the likely lab technique used to identify
this nucleotide change?
AG GGAAAC
...exon...AG GTAAGT...intron...
Range
7-9
9- 13
15- 21
15- 23
16-28
19- 28
20-36
20-38
wt/wt
db/db
consensus
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