QUESTION 1 Use these pka values to calculate the charge on the peptide below at pH 6. alpha COOH 2.0 side Chain COOH 4.4 His 7.0 Tyr 10.0 alpha amino 9.0 Lys 10 Arg 12 Cys 8 TSYMCFHKWGPRIE The charge on this peptide at pH 6 is and the pl of the peptide is

Organic And Biological Chemistry
7th Edition
ISBN:9781305081079
Author:STOKER, H. Stephen (howard Stephen)
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Chapter9: Proteins
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QUESTION 1
Use these pka values to calculate the charge on the peptide below at pH 6.
alpha COOH 2.0 side Chain COOH 4.4 His 7.0 Tyr 10.0 alpha amino 9.0 Lys 10 Arg 12 Cys 8
TSYMCFHKWGPRIE
The charge on this peptide at pH 6 is
and the pl of the peptide ia
QUESTION 2
How many cleavage products would be produced by cleavage of the peptide below with Trypsin
Which chromatography techniques would be best to separate the cleavage products
For Blank 2
STGIWKFESMLA
QUESTION 3
How many deavage products would be produced by cleavage of the peptide below with Chymotrypsn
Which chromatography techniques would be best to separate the cleavage products
STGIWKFESMLA
QUESTION 4
How many cleavage products would be produced by cleavage of he pepade below wth hermolyein
Which chromatograchy techques would be best to separate the cleavage products
STOIWKFESMLA
Transcribed Image Text:QUESTION 1 Use these pka values to calculate the charge on the peptide below at pH 6. alpha COOH 2.0 side Chain COOH 4.4 His 7.0 Tyr 10.0 alpha amino 9.0 Lys 10 Arg 12 Cys 8 TSYMCFHKWGPRIE The charge on this peptide at pH 6 is and the pl of the peptide ia QUESTION 2 How many cleavage products would be produced by cleavage of the peptide below with Trypsin Which chromatography techniques would be best to separate the cleavage products For Blank 2 STGIWKFESMLA QUESTION 3 How many deavage products would be produced by cleavage of the peptide below with Chymotrypsn Which chromatography techniques would be best to separate the cleavage products STGIWKFESMLA QUESTION 4 How many cleavage products would be produced by cleavage of he pepade below wth hermolyein Which chromatograchy techques would be best to separate the cleavage products STOIWKFESMLA
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