Ornithine is an amino acid that is not used in the synthesis of proteins, but is an important intermediate in several metabloic pathways including the urea cycle and the synthesis of polyamines. It has a perfectly ordinary terminal amino group and terminal carboxyl group like any other amino acid (so use the pKas for those groups given on your amino acids handout), and a side chain with a single ionizable side group with a pKa of 10.3. If ornithine is placed in solution at pH 7.0, it has a net charge of +1. What would the net charge on this amino acid be if the pH of the solution was raised to pH 12.0? Please explain your reasoning.

Q: A typical amino acid with one amino group and one carboxylicacid group, such as serine, can exist in…

A: At lower pH that means in acidic conditions the carboxylate ion takes up proton and exists as…

Q: Given pKa values for ionizable groups from Table 27.2, sketch curves for the titration of Q.)…

A:

Q: Use the values provided below to calculate the predicted isoelectric point, pl, value for aspartame…

A: Isoelectric point:  Isoelectric point of a amino acid is the pH at which amino acid has a neutral…

Q: You have been given a mixture of lysine, histidine and cysteine. The isoelectric point of the amino…

A: The mixture of amino can be separated by electrophoresis technique. This method is based on pH…

Q: At very low pH, alanine is a diprotic acid that can be represented as H3N1-CH(CH3)-COOH. The pKa of…

A: A diprotic acid contains two types of acidic groups. The amino acid contains these two groups: 1.…

Q: One of the most advanced methods for determining a protein sequence is tandem mass spectrometry…

A: Tandem mass spectroscopy includes the coupling of two or more mass analyzers to significantly…

Q: Hemoglobin A has a pI value of 5.6 whereas the variant hemoglobin M has an aspartate residue in…

A:

Q: What is the net charge on the tripeptide His-Glu-Lys at pH 7? The table below gives the pKa's of the…

A: Apart from the main -NH2 and -COOH groups, histidine has a side group (imidazole ring) with pKa =…

Q: a) Canavanine is an amino acid that is produced by some legume plants. It is not used in proteins…

A: Canavanine has a similar structure of Arginine.  Hence Canavanine has 3 groups that can be…

Q: Use three-letter amino acid abbreviations to give the sequence of amino acids in this polypeptide.…

A: Answer:- This question is answered by using the simple concept of naming of a polypeptide using the…

Q: The amino acid His is triprotic with pK1 (α-COOH) = 1.82, pK2 (α-NH3+) = 9.17, and pK3 (side chain)…

A: If the pKa of acid is equal or lower than the pH of the solution then the acid will get deprotonated…

Q: The amino acid histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, COOH H₂N-CH CH…

A: GIVEN The pKa1, pKa2 and pKa3 values of Histidine are given as 1.8, 6.0 and 9.2. Initial pH of…

Q: Another strategy used to resolve amino acids involves converting the carboxy group to an ester and…

A: Two enantiomers of alanine amino acid are present i.e. S & R form of it.

Q: Explain why the pI of lysine is the average of the pKa values of its two protonated amino groups.

A: First the lysine structure is noted as follows,

Q: Explain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a…

A: (a)  Comparing the structure of aspartate and serine as shown. Aspartate has the carbonyl group…

Q: decapeptide has the following amino acid composition: Ala2 , Arg, Cys, Glu, Gly, Leu, Lys, Phe, Val…

A: The tripeptide has lysine as N-terminal. Now writing all these tripeptide in such a manner that…

Q: What is the charge of each amino acid residue in the peptide chain and what is the net charge on the…

A: The peptide KGL contains the following amino acids lysine glycine leucine

Q: Glutamic acid is a naturally occurring α-amino acid that contains acarboxy group in its R group side…

A: a) At pH=1 , that is in highly acidic medium, the amino group gains a proton and thus the positive…

Q: Give the amino acid sequence of each peptide using the fragments obtained by partial hydrolysis of…

A: Primary constituents of peptides are amino acids. Amino acid sequence with 2 to 50 residues are…

Q: (b) Describe how the charge of some amino groups in a protein might differ at pH 9.0 and pH 5.0.…

A: Polypeptides are polymers of amino acids that make up proteins. The amino (–NH2) and carboxylic acid…

Q: The amino acid histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, as shown. COOH…

A:

Q: Draw a formula for Thr-Ala-Ala (T-A-A) in its predominant ionic form at pH 7.3. You may assume for…

A: Here we are required to draw the structure of peptide predominant at pH 7.3

Q: The isoelectric point (pl) of a peptide is the pH at which the Group pK peptide ceases to migrate in…

A:

Q: Consider a histidine residue within a protein. Based on the histidine side-chain pKa value of 6.0…

A: Histidine is a basic amino acid used to make up proteins which contain an amino group and a…

Q: The first step in the catabolism of most amino acids is the removal of the nitrogen atom by transfer…

A: Organic reactions are those in which organic reactant react to form organic products.

Q: 5 (a) Describe in detail how you will determine the primary structure of protein. (c) You have been…

A: Amino acids are those hydrocarbons that have a central carbon atom which is attached to a carboxylic…

Q: ogen pept is rigid, take place about the N-Co and the Co-C bonds in a protein (Co is the a carbon…

A:

Q: Give the amino acid sequence of each peptide using the fragments obtained by partial hydrolysis of…

A: The peptide linkage is formed between the amino group of one amino acid and the carboxyl group of…

Q: ine is a diprotic amino acid with a pk = 2.344 for the carboxylic group and a pK, = 9.868 for the…

A:

Q: You have been given a mixture of lysine, histidine and cysteine. The isoelectric point of the amino…

A: Electrophoresis- Separation of charged particles with the help of electric field. It consists of…

Q: The pKa values of an amino acid are the following: pK1 = 2.18;  pK2 = 8.95;  pK3 = 10.53, what is…

A: Tha pKa values indicates ;  pK1 = 2.18 ( this is the pKa of acidic group)  pK2 and pK3 are the pKa…

Q: Why is glycine the only amino acid commonly observed in peptides inconformations with phi and psi…

A:

Q: The amino acid sequence shown below is a short excerpt (residues 130-172) from the rod domain of an…

A: Following the rules of conservative amino acids, the amino acids that when replaced with a…

Q: Valine has pKa's of 2.286 and 9.719. Estimate the fractional composition of Valine in the -1 form at…

A: Amino acids are amphoteric in nature due to the presence their ionizable α-amino and α-carboxylic…

Q: Determine the amino acid sequence of a polypeptide from the following data:Complete hydrolysis of…

A:

Q: Calculate the isoelectric point for below amino acid. asparagine: pKa (COOH) = 2.02; pKa (α-NH3+) =…

A: Given: pKa (COOH) = pKa1 = 2.02 pKa (α-NH3+) = pKa2 = 8.80 Isoelectric point (pI) is simply equal to…

Q: Determine the amino acid sequence of a polypeptide from the following data: Complete hydrolysis of…

A: We can use the above given info to write the primary structure of the original polypeptide. First we…

Q: Given the chart of pKa’s below, calculate the isoelectric point for tyrosine

A: Isoelectric point (pI):The isoelectric point is the pH of an aqueous solution of an amino acid (or…

Q: Glycine is a diprotic acid, which can potentially undergo two dissociation reactions, one for the…

A:

Q: Draw the product that valine forms when it reacts with di-tert-butyl dicarbonate and triethylamine…

A: Here

Q: Alanine is a diprotic amino acid with a pk, = 2.344 for the carboxylic group and a pka = 9.868 for…

A: Ajj

Q: Consider the amino acid and its pKa values: Lysine: pKa1= 2.18 pKa2= 8.95, pKaR=10.79 a. Calculate…

A: Since you have posted question with multiple sub-parts, we are entitled to answer the first 3.

Q: A compound is known to have a free amino group with a pKa of 8.8, and one other ionizable group with…

A: Given compound is having two ionisable groups.

Q: The pKa for the side chain amino group in lysine is 10.4. If we were working at physiological pH…

A: At pH below the pKa for the amino acid is  protonated and at  pH above the pKa then it is…

Q: You have a peptide with the sequence HEEDRKYYLCG. You may assume that the side-chain pKa values from…

A: At pH above the pKa value of a particular group of an amino acid, the group gets deprotonated. At pH…

Q: The isoelectric point (pl) of phenylalanine is pH 5.5. Draw the structure of the major form of…

A:

Q: 4. Another peptide fragment that is 33 residues long is recognized as the most resistant to being…

A: In the given sequence of peptide, L, Q, P, F, and Y are five different amino acids.

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.