QUESTION 2 In order to compare transcript A and B expression levels in the same libarary based on their read/fragment counts, which of the following factors must be considered: 01.The number of fragments belong to each gene in the library O2 All of the O 3. Total number of fragments in the library (aka, sequencing depth) 04 Length of transcripts
QUESTION 2 In order to compare transcript A and B expression levels in the same libarary based on their read/fragment counts, which of the following factors must be considered: 01.The number of fragments belong to each gene in the library O2 All of the O 3. Total number of fragments in the library (aka, sequencing depth) 04 Length of transcripts
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:QUESTION 2
In order to compare transcript A and B expression levels in the same libarary based on their read/fragment counts,
which of the following factors must be considered:
O 1. The number of fragments belong to each gene in the library
02 All of the above.
3. Total number of fragments in the library (aka, sequencing depth)
O4 Length of transcripts
QUESTION 3
Which of the following quantification methods is the best way for comparison between
samples?
O 1. raw read count
2. RPKM
O 3. FPKM
O 4. TPM
QUESTION 4
What's the best way to deal with the reads/fragments which are mapped to multiple
transcripts or genome locations?
O 1. discard this read/fragment
O 2. evenly distribute this read/fragment to all the possible locations/transcripts
O 3. randomly assign this read/fragment to one of the possible location/transcript
4. Using Expectation-Maximization method to estimate the probability of the fragments to be mapped in each possible
location/transcript
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