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- #2. Solution needed How might we, as epidemiologists, increase the validity of genetic studies? What recommendations do you have (assume that you have all of the resources you'd need to implement these changes)?.Question with regards to SDS-PAG You are working with a unique protein that has no basic amino acids and contains a lot of acidic amino acids. You run a SDS-PAGE gel, you stain and destain it. You get nice bands for your ladder but no bands for your sample proteins. After doing an immunoblot specific for your protein (using the SDS-PAGE gel), you do get a band identifying your protein. You know that your concentration of the protein is sufficient to be visualized by staining it. The immunoblot clearly identifies that your protein is in your gel, why can you not see it after staining and destaining the gel?A MLS supervisor is writing an Standard Operating Procedure (SOP) for running a PC test for Hepatitis B DNA. There are several things she must include in the instructions to run the test.1. On average, how many PCR cycles must be run to see if the test is positive or negative for HBV?2. What would a positive test and a negative test look like?3. Her younger sister wants to know what she did at work today. How should she explain what happens during each step of the PCR cycle.
- QUESTION 4The ability of a bacterial isolate to perform hemolysis can be regarded as an indication ofpotential pathogenicity. Briefly describe how you would test for this trait. QUESTION 5You are asked to recommend an antibiotic to treat this infection in an animal. Describe amethod which will allow you to make this recommendation. QUESTION 6The isolate you obtained appears to be very unique. Another laboratory has requested a sampleof the isolate for further research. To transport the isolate, you need to transfer it from an agarplate to a transport media broth. Describe the procedure you would use.Topic: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting factors) Questions: Give a research scientist (not a company) who laid the foundation for this process OR is currently working in an area involving this process.HBs Antibody Principle: This test uses the “sandwich principle”, a slide phase colloidal gold enhanced immunoassay technique for determination of HBs antibody in human serum or plasma. The nitrocellulose membrane was immobilized with HBs antigen on the test band region and anti-HBsAg antibody on the control band region. During the assay, the specimen is allowed to react with the colored conjugate (HBs Ag colloid gold conjugate); the mixture then migrates chromatographically on the membrane by the capillary action. For a positive result, a color band with the specific antibody-HBsAg complex will form on the membrane. Absence of this colored band in the test band region suggests a negative result. To serve as a procedural control, a colored band at control region always appears in the test area. The reagent contains scavenge antibodies to reduce nonspecific reactivity in human serum or plasma specimens. The sensitivity of the test is 10mIU. HBs Antigen Principle:…
- 3 types of common coronavirus tests (RT-PCR for viral RNA with Taqman detection, lateral flow antibody tests, and lateral flow antigen tests) have been examined. a) The RT-PCR with Taqman detection is used to test for viral RNA in univeristy laboratory for testing saliva samples. Why do you think a univerisity chose chose this test to screen students/faculty/staff for Covid-19 on a regular basis? Explain your answer by discussing advantages/disadvantages of the test. b)An antigen test was used to screen students in when they arrived on campus. Why do you think a univeristy chose this test for this purpose? What are advantages and disadvantages of this test? c)Do you think the RT-PCR test with Taqman detection for viral RNA is best test for regular screening? Justify your answer.im a bit confused on the answer I was given for question 4, I believe the question is asking to discuss how genomic analysis would inform therapeutic choices, I am specifically looking for some therapy choices for malignant melanoma and to link them to the genomic analysis givenHuman Immunodeficiency Virus (HIV) Principle: The assay starts with a sample applied to the sample well. A recombinant HIV antigen conjugated to colloidal gold embedded in the sample pad reacts with the HIV antibody present in serum or plasma forming conjugate / HIV antibody complex. As the mixture is allowed to migrate along the test strip, the conjugate / HIV antibody complex is captured by recombinant HIV antigen immobilized on a membrane forming a colored test band in the test region. A negative sample does not produce a test band due to the absence of colloidal gold conjugate / HIV antibody complex. The antigens used in the conjugate test are recombinant proteins that correspond to highly immunoreactive regions of HIV 1 and HIV 2. A colored control band in the control region appears at the end of test procedure regardless of test result. This control band is the result of colloidal gold conjugate binding to the anti-HIV antibody immobilized on the membrane. The…
- Preimplantation genetic testing – aneuploidy (PGT-A) is a rapidly growing area of fertility research commonly incorporated into IVF research workflows. Explain in detail the methodology and technique used for Preimplantation Genetic Testing with Ion ReproSeq test? What other genetic tests could be recommended?Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicityA student said, “The approach shown by the Chicago researchers is just conventional gene therapy with biomaterial carrier”. Is he or she correct? Why? based on “Targeted polyelectrolyte complex micelles treat vascular complications in vivo”, PNAS 2021, Vol. 118 No. 50 e2114842118;107:110210; https://doi.org/10.1073/pnas.2114842118