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- Question 1: Suppose you have given 5 tablets weighing 449mg, 450mg, 448mg, 451mg and 453mg, respectively. Calculate 300 mg equivalent sample weight for assay. The product claim is 500 mg Vitamin C is present in each tablet. Question 2: Volume of the standard sodium edetate is 12.6 mL (calculated after the assay of Zinc Sulfate Syrup). Determine the % of potency of the drug considering the claimed dose is 11.5Question 2 You are checking a calculation that a foundation pharmacy student has performed. The question asks that 120 suppositories weighing 1 gram are to be made, containing 300mg Zinc Oxide. The displacement value of ZnO is 4.7 in the base that is to be used. How many grams of base would be required? units - g Answer to 2 decimal places Amount of base required = theoretical amount – displaced amount Where; Displaced amount = (amount of drug in g/DV of drug).Question 6 Which of the following is the correct sequence for the aseptic transfer of a bacterial culture? - 1. 2. 3. 4. 5. 6. 7. Remove the lid of the media receiving the culture & flame the mouth of this tube. - 1. 2. 3. 4. 5. 6. 7. Flame the mouth of the culture tube. - 1. 2. 3. 4. 5. 6. 7. Insert the wire loop with the culture to inoculate the bacteria on or in the new media. - 1. 2. 3. 4. 5. 6. 7. Reheat the mouth of the culture tube and replace the cap. - 1. 2. 3. 4. 5. 6. 7. Sterilize the loop by heating it from base to tip uniformly orange-hot. - 1. 2. 3. 4.…
- Question 15 Which of the following agents used for routine hand hygiene in health care settings is most bactericidal and least irritating to the skin? Question 15 options: a) Alcohol-based handrub b) Chlorhexidine handwash c) Plain soap and water d) Antimicrobial soap and waterQuestion No. 2 Differentiate between Batch Fermentation and Fed-Batch Fermentation. Enlist the differences between crude media and defined media.‘Practical techniques used to identify unknown microorganisms’ You should include the types of tests you would use and how to interpret the results. You do not have to include a step-by-step guide to each of the practical techniques, but rather information could include background, reasoning and/or expected results.
- Question 6 Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the concentration of BSA in each sample and say if the standard curve is adequate for each of these samples? Why or why not? If not, what would you do to fix this? Show your calculations.Question 16 Within the context of infectious disease, which of the following specimens would be considered irretrievable? Question 16 options: a) Wound swab b) Blood sample for therapeutic drug monitoring c) Cerebrospinal fluid d) Urine sampleQuestion 1.Identify the various classes of weighing balance used in the pharmaceutical laboratory and give it ranges. Question 2.In your own words and explanation give out the tentative meaning of extemporated production or compounding
- Question 5 You spread a small amount of a water sample from the environment on an enriched media agar plate. After 24 hours, the plate is covered with many small yellow colonies. Does this plate contain a pure culture of bacteria? ○ No, because it came from the environment, there are lots of different organisms. ○ We know there are multiple species present, but each colony is a different species. ○ Yes, we know the plate contains a pure culture because there are individual colonies. ○ We do not know if the plate contains a pure culture or a mixed culture. or even if individual colonies are pure or mixed based on their appearance alone.QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that will be used to prepare and run the agarose gel? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question but answers should be in your own words. Please include a reference for any sources you used)Question 14 When quantifying the amount of lipid in milk, an aliquot of milk is mixed with organic solvent and spun in a centrifuge. Three layers are observed in the tube after centrifugation. In which layer would you expect to contain the lipid component of milk? Aqueous layer at the top White middle layer Bottom solvent layer Question 17 Which of the following are true of cardiolipin? Tick all correct answers. It has four hydrocarbon tails. It has three glycerol molecules present in the structure. It has two hydrocarbon tails. It is a phosphoglycerolipid. It has only one glycerol molecules present in the structure. Question 19 Which of the following molecules are common in the head groups of phospholipids? Tick all correct answers. lysine choline serine glucose ethanolamine galactose