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A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. Amax Ben Amax Jerry STD CURVE JERRY STD CURVE BEN Amax Ben Amax Jerry CONC (mM) ABS ABS CONC (mM) ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457

A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. Amax Ben Amax Jerry STD CURVE JERRY STD CURVE BEN Amax Ben Amax Jerry CONC (mM) ABS ABS CONC (mM) ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457

Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter28: Atomic Spectroscopy
Section: Chapter Questions
Problem 28.15QAP
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A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. STD CURVE BEN Amax Ben Amax Jerry STD CURVE JERRY Aтаx Ben Amax Jerry CONC (mM) CONC (mM) ABS ABS ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457
Transcribed Image Text:A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. STD CURVE BEN Amax Ben Amax Jerry STD CURVE JERRY Aтаx Ben Amax Jerry CONC (mM) CONC (mM) ABS ABS ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287 0.607 1600 1.457 0.499 1600 0.678 1.457
Question 24 Using the given data above, determine the following information: What is the concentration of Ben in the solution ? 393 mM 366 mM O 497 mM 468 mM
Transcribed Image Text:Question 24 Using the given data above, determine the following information: What is the concentration of Ben in the solution ? 393 mM 366 mM O 497 mM 468 mM
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