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Question 3. Your professor asks you to make a staining solution to stain and observe specimens under the microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X hematoxylin dye and 0.01 M calcium chloride (CaC12). In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds of pipets, cylinders and beakers to measure volumes. a) Describe how you make the staining solution? b) On your following trip to the histology lab, you realize that all the pipets to accurately measure volumes smaller than 1 ml are broken. What alternative method do you need to use to avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?

Question 3. Your professor asks you to make a staining solution to stain and observe specimens under the microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X hematoxylin dye and 0.01 M calcium chloride (CaC12). In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds of pipets, cylinders and beakers to measure volumes. a) Describe how you make the staining solution? b) On your following trip to the histology lab, you realize that all the pipets to accurately measure volumes smaller than 1 ml are broken. What alternative method do you need to use to avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 3. Your professor asks you to make a staining solution to stain and observe specimens under the microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X hematoxylin dye and 0.01 M calcium chloride (CaC12). In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds of pipets, cylinders and beakers to measure volumes. a) Describe how you make the staining solution? b) On your following trip to the histology lab, you realize that all the pipets to accurately measure volumes smaller than 1 ml are broken. What alternative method do you need to use to avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?
Transcribed Image Text:Question 3. Your professor asks you to make a staining solution to stain and observe specimens under the microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X hematoxylin dye and 0.01 M calcium chloride (CaC12). In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds of pipets, cylinders and beakers to measure volumes. a) Describe how you make the staining solution? b) On your following trip to the histology lab, you realize that all the pipets to accurately measure volumes smaller than 1 ml are broken. What alternative method do you need to use to avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?
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