Spectroscopy QUESTION 4Autozero (pressing Measure Blank) is required when a new wavelength is setO TrueO FalseQUESTION 5A spectrophotometer measuresO the rate of a enzyme reactionO the absorbance of lightthe concentration of unknown compoundsO the wavelengthQUESTION 6Which of the following wavelength ranges describes visible light?O 200-400 nmO 400-700 nmO 100-200 nmO 700-1000 nmClick Save and Submit to save and submit. Click Save All Ansuers to save all answers.to searchF2F5F6F9F10%23234 What are the two reasons for measuring the absorbance of a sample at its A max?O'A max is in the range of visible lightOBetter sensitivity to distinguish two similar concentrations of the same chemical compoundV Absorbance is more accurate, the error in determing concentration will be lowestV Better sensitivity to distinguish two different chemical compoundsQUESTION 2An absorption spectrum for KMNO4 will be generated in this experiment. What will be reported on the X- axis?O AbsorbanceWavelengthO TimeO ConcentrationQUESTION 3A standard curve for KMNO4 will be generated in this experiment. What will be reported on the X- axis?O AbsorbanceO ConcentrationO WavelengthO MinutesClick Save and Submit to save and submit. Click Save All Answers to save all answers.to searchF7F8F9F10F%233.7

3. Spectrophotometer needs to be zeroed (blanked) to reset its absorbing calibration to any…

QUESTION 4 Autozero (pressing Measure Blank) is required when a new wavelength is set O True O False QUESTION 5 A spectrophotometer measures the rate of a enzyme reaction O the absorbance of light O the concentration of unknown compounds O the wavelength QUESTION 6 Which of the following wavelength ranges describes visible light? 200-400 nm 400-700 nm 100-200 nm O 700-1000 nm

QUESTION 4 Autozero (pressing Measure Blank) is required when a new wavelength is set O True O False QUESTION 5 A spectrophotometer measures the rate of a enzyme reaction O the absorbance of light O the concentration of unknown compounds O the wavelength QUESTION 6 Which of the following wavelength ranges describes visible light? 200-400 nm 400-700 nm 100-200 nm O 700-1000 nm

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 5Q

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Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/L
You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2
You have carried out an experiment using the spectrophotometry concept. A solutioncontaining compound X is mixed with reagent 1 and then reagent 2. This mixture produces ablue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM:1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).
The electronic excitation method in fluorescence and absorption spectroscopy is the same. What are some of the variations between using a fluorimeter to perform an excitation scan and an absorption scan? Do you think the two spectra should be similar? Describe. More info attached like protocol if needed
For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.
After performing the manual Albumin assay, you get the absorbance value of 0.205 for a 4.5 g/dL albumin standard and 0.114 for control A. What is the calculated value of the control? (just write a number with one decimal, the units will be mg/dL)
A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
The material to be heated is in a long, cylindrical tube, and is viscous enough that any flow inside the tube can be ignored, i.e. the heating is by conduction alone). Also, consider the glass of the tube to be very thin so its thermal resistance can be ignored. The tube diameter is 1 cm and the properties of the material are the same those of water, given by 0.64 W/m*K, 1000 kg/m3, and 4180 J/kg*K, for thermal conductivity, density, and specific heat, respectively. The water bath temperature is 65 °C, and the initial temperature of the material is 27 °C. Without the agitation of the water bath, the heat transfer coefficient can be considered to be 128 W/m2*K. Considering the tube to be a long cylinder, how long does it take for the coldest point to heat up to a temperature corresponding to 99% of the total possible temperature increase? If the surrounding how water is agitated using a stirrer, leading to a considerably large surface heat transfer coefficient, how long does it…
1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.
An IV bag of NS containing 30 g KCl is being administered at a rate of 80 mL/hr using a macrodrip tubing with a DF of 15 gtts/mL. How many drops per minute is that? Group of answer choices a. 30 gtts/min b. 22 gtts/min c. 10 gtts/min d. 20 gtts/min
For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?