Question 5: (a) Why is it important that gluconeogenesis is not the exact reversal of glycolysis? (b) A liver extract capable of carrying out all the normal metabolic reactions of the liver is briefly incubated with "C-labelled bicarbonate ion, ["C]-HCO;. Trace the pathway of this labeled precursor through gluconeogenesis and indicate the location of the "C label in all intermediates and in the product glucose.
Q: || CH3CSCOA "02CCH2CSCOA
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![Question 5: (a) Why is it important that gluconeogenesis is not the exact reversal of
glycolysis? (b) A liver extract capable of carrying out all the normal metabolic reactions
of the liver is briefly incubated with "C-labelled bicarbonate ion, [1"C]-HCO,. Trace the
pathway of this labeled precursor through gluconeogenesis and indicate the location of
the "C label in all intermediates and in the product glucose.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F79b6cd97-0699-49d2-a2d6-510dbc9b8302%2F6afaa2ee-7467-43c2-8ed8-0332f0e1ecb6%2Fnrlntgr_processed.jpeg&w=3840&q=75)
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- QUESTION 22 When the final product of a series of enzymatically-catalyzed reactions binds to the first enzyme in the pathway to limit its production, it generally uses ___ because the structure of this final product is generally not similar to that of any of the enzyme's normal substrates. Allosteric activation Zymogen activation Covalent modification Competitive inhibition Allosteric inhibitionQuestion: A. To explore the consequences of coupling ATP hydrolysis under physiological conditions to a thermodynamically unfavorable biochemical reaction, consider the hypothetical transformation X⟶Y, for which Δ?′°=20.0 kJ/mol. What is the ratio of [Y]/[X][Y]/[X] at equilibrium? B. Suppose XX and YY participate in a sequence of reactions during which ATP is hydrolyzed to ADP and Pi. The overall reaction is X+ATP+H2O⟶Y+ADP+Pi Calculate [Y]/[X] for this reaction at equilibrium. Assume that the temperature is 25.0 °C and the equilibrium concentrations of ATP, ADP, and Pi are 1.00 M each. C. We know that [ATP], [ADP], and [Pi] are not 1.00 M under physiological conditions. Calculate [Y]/[X] for the ATP‑coupled reaction when the values of [ATP], [ADP], and [Pi] are those found in rat myocytes. Metabolite Concentration in rat myocytes (M) ATP 8.05x10-3 ADP 0.93x10-3 Pi 8.05x10-3Question 1: In some microorganisms, carbon fixation occurs by reversal of the citric acid cycle. This reversal is accomplished in part by the use of a strong reductant (ferredoxin) to drive the alpha-ketoglutarate dehydrogenase reaction in the reductive direction. Part a: ΔG°‘ for reaction as it occurs in the ‘normal’ (oxidative) citric acid cycle is -30.1 kJ/mol. The standard reduction potential for NADH is -0.32 V. In order to drive the reaction in the reverse direction, the reductant (a ferredoxin) must have a lower reduction potential than NADH/NAD+. Remembering that this is a two-electron reduction, and using the numbers given just above, compute the value of the ferredoxin reduction potential that would be needed to make the standard free energy zero (so that the reductive reaction is enegetically just as favorable as the oxidative reaction). Assume that all of the other reactants are the same in the reductive as in the oxidative reaction. Write out the steps in your calculation;…
- QUESTION 26 During gluconeogenesis, whereby liver cells convert pyruvate to glucose, Fructose-6-phosphate (F6P) is converted to Glucose-6-phosphate (G6P). If the standard equilibrium concentrations are: [F6P] = 0.52 M and [G6P] = 1.48 M, then Keq’ is ______ and the reaction is ________. Fructose-6-P ó Glucose-6-P > 1; exergonic > 1; endergonic < 1; exergonic < 1; endergonicQUESTION 1 a. John has just taken his Lunch meal, after which he is going to takes his Daily soccer practice match in the next 30 min, how will glycolysis operate/work in such conditions or be applicable b. How will oxidative phosphorylation work in sickly John who usually practice daily in soccer? Make possible scenarios c. Enzymes are critical in the metabolism of most most organic compounds, actually 30% of reactions are catalysed by them. How would the inactive enzymes (Sickness) in electron Transport Chain compromise the fat breakdown in the body.Question 1: The 4 kinase steps in glycolysis are catalyzed by hexokinase, phosphofructokinase, phosphoglycerate kinase, and pyruvate kinase. Write each of these four reactions, including all participants. i. ii. iii. iv.
- QUESTION 2 2.1 Draw a metabolic chart indicating the carbohydrate transporters involved in intestinal transport and distribution of glucose, ga/actose and fructose. 2.2. Draw a metabolic chart depicting the reactions of phase 1 of the glycolysis pathway, including metabolite names, enzyme names and cofactors. Chemical structures are not a requirement in this answer 2.3.raw a diagram of the reactions of mitochondria/ 8-oxidation of hexanoic acid (CH3-(CH2)4-COOH). Include activation and mitochondria/ transportation and the structures of the intermediatesQuestion 1: Part a: Assume that the standard free energy of ATP hydrolysis is -31 kJ/mol. Assume the following values for the standard free energy changes of the four reactions: HK -16.7 kJ/mol; PFK -14.2 kJ/mol; PGK -18.9 kJ/mol; PK -31.7 kJ/mol. (from bio.libretexts.org). Use these values to compute the standard free energy of hydrolysis (releasing Pi) of i. glucose 6-P ii. fructose 1,6-bis-P iii. 1,3-bisphosphoglycerate iv. phosphoenolpyruvate Part b: Which of these four compounds is the strongest phosphoryl donor?______________ Which is the weakest?__________________ Part c: The phosphoglycerate kinase reaction is favorable by -18.9 kJ/mol in the glycolytic direction, as stated above. In gluconeogenesis, this step is simply reversed; i.e. it is not one of the three steps in gluconeogenesis that is driven by using different chemistry than that of glycolysis. How can this be? (Be specific: what specific factors could enable reversal of this step?)Nearly all organisms on Earth carry out some form of glycolysis. How does this fact support or not support the assertion that glycolysis is one of the oldest metabolic pathways?
- Question:- 33) Glucose-6-phosphate has different pathways that it can enter in the liver during the fed state. All of the following are possible except OA.) it is oxidized to acetyl CoA for fatty acid biosynthesis O B). it can enter the pathway for glycogen biosynthesis O c.) it is a substrate for glucose-6-phosphatase OD. )it is oxidized in glycolysis to provide energy for the bodyQUESTION 1: What fatty acid can cross membranes as free fatty acid without protein assistance, could then become substrate of beta-oxidation in mitochondria, and the produced acetyl-CoA could end being used for histone acetylation in the nucleus?Question No. 1 Give the advantages and disadvantages of submerged fermentation and solid state fermentation. Give two examples of acetic acid fermentation.