Question 5 An initial sequencing test revealed that the usd1 mutant contains many noise mutations besides the true mutation, what would be the best way to clear these noise mutations? | She could outcross the usd1 mutant to another ecotype to clean the background. | She could increase the F2 mutant populations to narrow down the causative mutations. She could increase the sequencing depth from 50 times to 100 times of her libraries of the F2 mutants to

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 5
An initial sequencing test revealed that the usd1 mutant contains many noise mutations besides
the true mutation, what would be the best way to clear these noise mutations?
She could outcross the usd1 mutant to another ecotype to clean the background.
She could increase the F2 mutant populations to narrow down the causative mutations.
She could increase the sequencing depth from 50 times to 100 times of her libraries of the F2 mutants to
narrow down the causative mutations.
She could backcross the usd1 mutant to the parent line to clean the background before outcross
Transcribed Image Text:Question 5 An initial sequencing test revealed that the usd1 mutant contains many noise mutations besides the true mutation, what would be the best way to clear these noise mutations? She could outcross the usd1 mutant to another ecotype to clean the background. She could increase the F2 mutant populations to narrow down the causative mutations. She could increase the sequencing depth from 50 times to 100 times of her libraries of the F2 mutants to narrow down the causative mutations. She could backcross the usd1 mutant to the parent line to clean the background before outcross
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