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Questions:
Which samples can have their own (innate) fluorescence?
Part of the spectrophotometer that transmits the desired wavelength: a.monochrome b.Selector c.Detector d.prism
Step by step
Solved in 2 steps
- How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!Answer briefly and concisely. Thank you! What have you observed regarding the brightness of the light field when the objectives were shifted from a lower magnification to a higher magnification? What is the importance of a microscope in the study of plants and in drug discovery?Which of the ff. best describes the configuration of a traditionalcolorimeter? a.) light source --> wavelength selector --> sample holder --> wavelength detector --> photon detectorb.) light source --> sample holder -> wavelength detector -> photon detectorc.) light source --> wavelength selector --> sample holder --> photon detectord.) light source --> wavelength selector --> sample holder --> wavelength detector
- Based on your observations: Please answer in a full sentence. 1. At what magnification you can see the largest area on your slide? 2. At what magnification you can see the smallest FOV on the slide? 3. What happens to the light intensity as you increase the magnification? 4. What is the proper way to focus a microscope?Contrast how the views differ under high power (40x) and oil immerson (100x) objective lenses in terms of a) magnification b) detail of the cell, c) size of the area being viewed and d) resolution.Which of the following will decrease the limit of resolution (Select all that applies) a) using lower wavelength of illumination b) using an objective lens with lower numerical aperature c) using immersion oil d) using electron as the source of illumination instead of light
- How is the UV spectrometer work? What is absorbance and how is it determined?What is the proper method of cleaning the microscope? Select an answer. a. Change the magnification to the lowest power, clean the objective lens and ocular lens with lens paper b. Change the magnification to the highest power, clean the objective lens and ocular lens with lens paper c. Change the magnification to the lowest power, clean the objective lens and ocular lens with a paper towelIf a light microscope has a objective power of 150x and an ocular power of 10x, what is the total magnification? Question options: 15 Not enough information to tell 140 1,500 160 150 15,000
- Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceCan colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?Explain how total magnification is calculated. Select an answer. a. It is the magnification of the objective lens multiplied by the magnification of the condenser lens b. It is the magnification of the objective lens multiplied by the magnification of the ocular lens c. It is the magnification of the objective lens used d. It is the magnification of the ocular lens used
